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dc.contributor.author
Awruch, Cynthia Andrea
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dc.contributor.author
Simpfendorfer, C.
dc.contributor.author
Pankhurst, N. W.
dc.date.available
2020-01-03T20:39:00Z
dc.date.issued
2011-06
dc.identifier.citation
Awruch, Cynthia Andrea; Simpfendorfer, C.; Pankhurst, N. W.; Evaluation and use of a portable field kit for measuring whole-blood lactate in sharks; Csiro Publishing; Marine and Freshwater Research; 62; 6; 6-2011; 694-699
dc.identifier.issn
1323-1650
dc.identifier.uri
http://hdl.handle.net/11336/93504
dc.description.abstract
Measurement of lactate is becoming a common procedure in assessing the physiological effects of capture stress in sharks, although the necessity to measure the concentrations in the laboratory limits the ability for field assessments. Portable lactate analysers offer an alternative, but await validation against laboratory assays for sharks. The present study assessed the reliability of a portable Lactate Pro analyser for measuring whole-blood lactate in the school shark, Galeorhinus galeus, in the field. Laboratory determination of whole-blood and plasma lactate obtained by spectrophotometry was highly correlated with field determinations. Because shark lactate concentration can exceed the upper detection limit of the portable analysers, which were designed for mammalian use, a method for dealing with values greater than the maximum detection limit was evaluated. Whole-blood diluted by 50% with acidified saline solution, tap water and distilled water gave measured values of 55, 56 and 52%, respectively, of the original values, allowing accurate estimation of concentrations exceeding the upper detection limit of the analyser. These findings indicated that the Lactate Pro can be used to rapidly and reliably measure lactate for sharks in the field.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Csiro Publishing
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ELASMOBRANCHS
dc.subject
PLASMA LACTATE
dc.subject
PORTABLE ANALYSER
dc.subject
STRESSORS
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Evaluation and use of a portable field kit for measuring whole-blood lactate in sharks
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-08-27T18:45:46Z
dc.journal.volume
62
dc.journal.number
6
dc.journal.pagination
694-699
dc.journal.pais
Australia
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dc.journal.ciudad
Collingwood
dc.description.fil
Fil: Awruch, Cynthia Andrea. James Cook University; Australia
dc.description.fil
Fil: Simpfendorfer, C.. James Cook University; Australia
dc.description.fil
Fil: Pankhurst, N.W.. Griffith University; Australia
dc.journal.title
Marine and Freshwater Research
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1071/MF10149
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/mf/MF10149
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