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dc.contributor.author
Chiarini, Franco Ezequiel
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dc.date.available
2016-12-13T21:21:59Z
dc.date.issued
2014-05
dc.identifier.citation
Chiarini, Franco Ezequiel; Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae); Rsnz Publishing; New Zealand Journal Of Botany; 52; 3; 5-2014; 277-284
dc.identifier.issn
0028-825X
dc.identifier.uri
http://hdl.handle.net/11336/9318
dc.description.abstract
Fluorescence in situ hybridization (FISH) was applied to detect gene rearrangements and genome downsizing in Solanum elaeagnifolium, an invasive weed with 2n = 24, 48 and 72 populations. The probes employed were pTa71 containing the 18S5.8S26S gene, and a 5S rDNA probe obtained from S. stuckertii. As a result, diploids presented a pair of 18S5.8S26S signals and a pair of 5S, whereas tetra- and hexaploids increased their FISH marks proportional to the ploidy level. In all cases, rDNA loci were non-syntenic, and position was conserved along the polyploid series. The cytotypes would have formed recently and drastic rearrangements have not yet occurred. However, in each cell of the 4x and 6x individuals, signal intensity in one locus of the 18S5.8S26S was stronger, suggesting a potential loss of copies in the remaining loci. In addition, one hexaploid showed an extra 5S signal in heterozygosis. The data obtained suggest a recent, recurrent autopolyploidy.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Rsnz Publishing
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Solanum Elaeagnifolium
dc.subject
Polyploidy
dc.subject
Rdna Loci
dc.subject
Genome Downsizing
dc.subject.classification
Genética y Herencia
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-12-12T14:18:11Z
dc.journal.volume
52
dc.journal.number
3
dc.journal.pagination
277-284
dc.journal.pais
Nueva Zelanda
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dc.journal.ciudad
Auckland
dc.description.fil
Fil: Chiarini, Franco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
dc.journal.title
New Zealand Journal Of Botany
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/0028825X.2014.888087
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/0028825X.2014.888087
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