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dc.contributor.author
Fernández Baldo, Martín Alejandro  
dc.contributor.author
Messina, Germán Alejandro  
dc.contributor.author
Sanz Ferramola, Maria Isabel  
dc.contributor.author
Raba, Julio  
dc.date.available
2019-12-24T00:09:19Z  
dc.date.issued
2010-11  
dc.identifier.citation
Fernández Baldo, Martín Alejandro; Messina, Germán Alejandro; Sanz Ferramola, Maria Isabel; Raba, Julio; Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits; American Chemical Society; Journal of Agricultural and Food Chemistry; 58; 21; 11-2010; 11201-11206  
dc.identifier.issn
0021-8561  
dc.identifier.uri
http://hdl.handle.net/11336/92876  
dc.description.abstract
A wide range of plant species, including economically important crops such as vegetables, ornamentals, bulbs, and fundamentally fruits, can be affected by gray mold caused by the fungal pathogen Botrytis cinerea. This paper describes the development of a microfluidic immunosensor with micromagnetic beads (MMBs) coupled to carbon-based screen-printed electrodes (SPCEs) for the rapid and sensitive quantification of B. cinerea in apple (Red Delicious), table grape (pink Moscatel), and pear (William's) tissues. The detection of B. cinerea was carried out using a competitive immunoassay method based on the use of purified B. cinerea antigens immobilized on 3-aminopropyl-modified MMBs. The total assay time was 40 min, and the calculated detection limit was 0.008 μg mL -1. Moreover, the intra-and interassay coefficients of variation were below 7%. The developed method allowed detects B. cinerea even in asymptomatic fruits and promises to be particularly useful for application in the agricultural industry.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
American Chemical Society  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
BOTRYTIS CINEREA  
dc.subject
ENZYME IMMUNOASSAYS  
dc.subject
FLOW INJECTION ANALYSIS  
dc.subject
HORSERADISH PEROXIDASE  
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MICROFLUIDIC SYSTEM  
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MICROMAGNETIC BEADS  
dc.subject.classification
Química Analítica  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-10-10T19:36:16Z  
dc.journal.volume
58  
dc.journal.number
21  
dc.journal.pagination
11201-11206  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Washington  
dc.description.fil
Fil: Fernández Baldo, Martín Alejandro. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; Argentina  
dc.description.fil
Fil: Messina, Germán Alejandro. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; Argentina  
dc.description.fil
Fil: Sanz Ferramola, Maria Isabel. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; Argentina  
dc.description.fil
Fil: Raba, Julio. Microfluidic Immunosensor with Micromagnetic Beads Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs) for Determination of Botrytis cinerea in Tissue of Fruits; Argentina  
dc.journal.title
Journal of Agricultural and Food Chemistry  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1021/jf1025604  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/jf1025604