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dc.contributor.author
Fernández Baldo, Martín Alejandro
dc.contributor.author
Messina, Germán Alejandro
dc.contributor.author
Sanz Ferramola, Maria Isabel
dc.contributor.author
Raba, Julio
dc.date.available
2019-12-24T00:09:19Z
dc.date.issued
2010-11
dc.identifier.citation
Fernández Baldo, Martín Alejandro; Messina, Germán Alejandro; Sanz Ferramola, Maria Isabel; Raba, Julio; Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits; American Chemical Society; Journal of Agricultural and Food Chemistry; 58; 21; 11-2010; 11201-11206
dc.identifier.issn
0021-8561
dc.identifier.uri
http://hdl.handle.net/11336/92876
dc.description.abstract
A wide range of plant species, including economically important crops such as vegetables, ornamentals, bulbs, and fundamentally fruits, can be affected by gray mold caused by the fungal pathogen Botrytis cinerea. This paper describes the development of a microfluidic immunosensor with micromagnetic beads (MMBs) coupled to carbon-based screen-printed electrodes (SPCEs) for the rapid and sensitive quantification of B. cinerea in apple (Red Delicious), table grape (pink Moscatel), and pear (William's) tissues. The detection of B. cinerea was carried out using a competitive immunoassay method based on the use of purified B. cinerea antigens immobilized on 3-aminopropyl-modified MMBs. The total assay time was 40 min, and the calculated detection limit was 0.008 μg mL -1. Moreover, the intra-and interassay coefficients of variation were below 7%. The developed method allowed detects B. cinerea even in asymptomatic fruits and promises to be particularly useful for application in the agricultural industry.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BOTRYTIS CINEREA
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ENZYME IMMUNOASSAYS
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FLOW INJECTION ANALYSIS
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HORSERADISH PEROXIDASE
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MICROFLUIDIC SYSTEM
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MICROMAGNETIC BEADS
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Química Analítica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (SPCEs) for determination of botrytis cinerea in tissue of fruits
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-10T19:36:16Z
dc.journal.volume
58
dc.journal.number
21
dc.journal.pagination
11201-11206
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Fernández Baldo, Martín Alejandro. Microfluidic Immunosensor with Micromagnetic Beads
Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs)
for Determination of Botrytis cinerea in Tissue of Fruits; Argentina
dc.description.fil
Fil: Messina, Germán Alejandro. Microfluidic Immunosensor with Micromagnetic Beads
Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs)
for Determination of Botrytis cinerea in Tissue of Fruits; Argentina
dc.description.fil
Fil: Sanz Ferramola, Maria Isabel. Microfluidic Immunosensor with Micromagnetic Beads
Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs)
for Determination of Botrytis cinerea in Tissue of Fruits; Argentina
dc.description.fil
Fil: Raba, Julio. Microfluidic Immunosensor with Micromagnetic Beads
Coupled to Carbon-Based Screen-Printed Electrodes (SPCEs)
for Determination of Botrytis cinerea in Tissue of Fruits; Argentina
dc.journal.title
Journal of Agricultural and Food Chemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1021/jf1025604
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/jf1025604
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