Sequence and Expression of Two cry8 Genes from Bacillus thuringiensis INTA Fr7-4, a Native Strain from Argentina

We found and characterized two cry8 genes from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. These genes, cry8Kb3 and cry8Pa3 , are located in a tandem array within a 13,200-bp DNA segment sequenced from a preparation of total DNA. They encode 1,169- And 1,176-amino-acid proteins, respectively. Both genes were cloned with their promoter sequences and the proteins were expressed separately in an acrystalliferous strain of B. thuringiensis leading to the formation of ovoid crystals in the recombinant strains . The toxicity against larvae of Anthonomus grandis Bh. (Coleoptera: Curculionidae) of a spore-crystal suspension from the recombinant strain containing cry8Pa3 was similar to that of the parent strain INTA Fr7-4.

Palabras clave: Bacillus thuringiensis , Cry8 , Expression

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)

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URI: http://hdl.handle.net/11336/92827

URL: http://www.karger.com/Article/FullText/365929

DOI: http://dx.doi.org/10.1159/000365929

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Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE

Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL

Citación

Navas, Laura Emilce; Berretta, Marcelo Facundo; Perez, Melisa Paula; Amadio, Ariel Fernando; Ortiz, Elio Maximiliano; et al.; Sequence and Expression of Two cry8 Genes from Bacillus thuringiensis INTA Fr7-4, a Native Strain from Argentina; Karger; Journal of Molecular Microbiology and Biotechnology; 24; 4; 11-2014; 241-248

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