Artículo
Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae)
Attademo, Andres Maximiliano
; Peltzer, Paola
; Lajmanovich, Rafael Carlos
; Basso, Agustin; Junges, Celina Maria
Fecha de publicación:
02/2014
Editorial:
Springer
Revista:
Water, Air and Soil Pollution
ISSN:
1567-7230
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We determined basal levels of cholinesterase (ChE) and carboxylesterase (CbEs; two substrates: α- naphthyl acetate and 4-nitrophenylvalerate) in different tissues of tadpoles and adults of the frog Pseudis paradoxa and evaluated their use as complementary biomarkers of anti-cholinesterase pesticide exposure. ChE and CbEs sensitivity to malaoxon was also evaluated. Adults and tadpoles were collected with sweep net from temporary ponds located in natural riparian forests along the Paraná River (Garay Department, Santa Fe province, Argentina). We found significant differences in B-esterase activities between adults and tadpoles and among different tissues. The in vitro inhibition tests indicated that ChE is more sensitive to inhibition than CbEs. Our results suggest that basal ChE and CbE (α-NA and 4- NPV) activities in different tissues of adult and tadpoles P. paradoxa would be suitable biomarkers of pesticide exposure, and this amphibian species could be used as sentinel in field monitoring.
Palabras clave:
CHOLINESTERASE
,
CARBOXYLESTERASE
,
MALAOXON
,
PSEUDIS PARADOXA
,
BIOMARKER
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Citación
Attademo, Andres Maximiliano; Peltzer, Paola; Lajmanovich, Rafael Carlos; Basso, Agustin; Junges, Celina Maria; Tissue-specific variations of esterase activities in the tadpoles and adults of Pseudis paradoxa (anura: Hylidae); Springer; Water, Air and Soil Pollution; 225; 3; 2-2014; 1903-1912
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