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dc.contributor.author
Garcia, Valeria Soledad
dc.contributor.author
Gonzalez, Veronica Doris Guadalupe
dc.contributor.author
Marcipar, Ivan Sergio
dc.contributor.author
Gugliotta, Luis Marcelino
dc.date.available
2016-12-13T17:30:38Z
dc.date.issued
2014-08
dc.identifier.citation
Garcia, Valeria Soledad; Gonzalez, Veronica Doris Guadalupe; Marcipar, Ivan Sergio; Gugliotta, Luis Marcelino; Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes; Wiley; Tropical Medicine And International Health; 19; 8-2014; 1346-1354
dc.identifier.issn
1360-2276
dc.identifier.uri
http://hdl.handle.net/11336/9267
dc.description.abstract
OBJETIVE To evaluate the diagnostic performance of novel latex–protein complexes obtained from different antigens of Trypanosoma cruzi through immunoagglutination test using a panel of T. cruzipositive sera, leishmaniasis-positive sera and negative sera for both parasites.
METHODS Complexes’ behaviour using total parasite homogenate (TPH), two simple recombinant proteins (RP1 and RP5) and two chimeric recombinant proteins (CP1 and CP2) was comparatively evaluated. The area under ROC curves was used as an index of accuracy. Sensitivity, specificity and discrimination efficiency were assessed.
RESULTS All recombinant antigens showed higher specificity than TPH. The lower specificity of TPH was mainly due to cross-reacting peptides between T. cruzi and Leishmania spp. In turn, all performance indicators were higher for CP1 and CP2 than for RP1 and RP5. The carboxylated latex– CP2 (C2–CP2) complex was able to detect antibodies against T. cruzi. The values of area under ROC curve (0.96), sensitivity (92.3%, 95% CI: 79.4–100.0%) and specificity (84.0%, 95% CI: 67.6– 100.0%) indicate that the assay could be used as a screening test. conclusion The C2–CP2 complex could be an important tool to carry out sero-epidemiological studies.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Immunoagglutination
dc.subject
Chagas Disease
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Latex-Protein Complexes
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Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-12-12T13:45:05Z
dc.journal.volume
19
dc.journal.pagination
1346-1354
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Garcia, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
dc.description.fil
Fil: Gonzalez, Veronica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
dc.description.fil
Fil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina
dc.description.fil
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
dc.journal.title
Tropical Medicine And International Health
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/tmi.12379
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/tmi.12379/abstract
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