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dc.contributor.author
Díaz Nieto, César Horacio  
dc.contributor.author
Granero, Adrian Marcelo  
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López, Jimena Claudia  
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Pierini, Gastón Dario  
dc.contributor.author
Levin, Gustavo Javier  
dc.contributor.author
Fernandez, Hector  
dc.contributor.author
Zon, María Alicia  
dc.date.available
2019-12-18T18:06:10Z  
dc.date.issued
2018-06  
dc.identifier.citation
Díaz Nieto, César Horacio; Granero, Adrian Marcelo; López, Jimena Claudia; Pierini, Gastón Dario; Levin, Gustavo Javier; et al.; Development of a third generation biosensor to determine hydrogen peroxide based on a composite of soybean peroxidase/chemically reduced graphene oxide deposited on glassy carbon electrodes; Elsevier Science Sa; Sensors and Actuators B: Chemical; 263; 6-2018; 377-386  
dc.identifier.issn
0925-4005  
dc.identifier.uri
http://hdl.handle.net/11336/92491  
dc.description.abstract
A third generation enzymatic biosensor is developed to determine H2O2. The biosensor is based on the use of a composite obtained from soybean peroxidase enzyme and chemically reduced graphene oxide deposited at glassy carbon electrodes. Experiments were carried out in 0.1 M phosphate buffer solutions, pH 7.0.Cyclic voltammograms of the biosensor show two reduction peaks centered at about 0.15 and −0.4 V, respectively. A quasi-reversible redox couple is defined in the region of potentials of the first peak, which is assigned to the reduction/oxidation of compound II involved in the catalytic cycle of peroxidases. The surface concentration of the electroactive enzyme was (1.1 ± 0.2) × 10−10 mol cm−2. Values of 0.33 and 0.04 s−1 were determined for the cathodic charge transfer coefficient and the heterogeneous electron transfer rate constant, respectively. UV–vis spectroscopy and scanning electron microscopy (SEM) were used to demonstrate the chemical reduction of graphene oxide. Electrochemical impedance spectroscopy was used to characterize the different stages in the process of the electrode surface modification. Amperometric measurements were performed at a potential of −0.090 V vs. Ag/AgCl. Current responses were linear in the concentration range from 1.5 × 10−7 to 3.0 × 10−6 M. The limit of detection, limit of quantification, reproducibility, and repeatability were 5 × 10−8 M, 1.5 × 10−7 M, 9%, and 4%, respectively. The biosensor was stable during five days. The Michaelis Menten apparent constant was 1.6 × 10−6 M. The presence of uric acid, glucose, dopamine and ascorbic acid do not interfere in the determination of H2O2. The biosensor was applied to the determination of H2O2 in commercial contact lens care solutions. Good accuracy and recovery parameters were obtained.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science Sa  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
BIOSENSOR  
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CHEMICALLY REDUCED GRAPHENE OXIDE  
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COMPOUND II  
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HYDROGEN PEROXIDE  
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SOYBEAN PEROXIDASE ENZYME  
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Química Analítica  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Development of a third generation biosensor to determine hydrogen peroxide based on a composite of soybean peroxidase/chemically reduced graphene oxide deposited on glassy carbon electrodes  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-10-23T21:35:46Z  
dc.journal.volume
263  
dc.journal.pagination
377-386  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Díaz Nieto, César Horacio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina  
dc.description.fil
Fil: Granero, Adrian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina  
dc.description.fil
Fil: López, Jimena Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina  
dc.description.fil
Fil: Pierini, Gastón Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina  
dc.description.fil
Fil: Levin, Gustavo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina  
dc.description.fil
Fil: Fernandez, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina  
dc.description.fil
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina  
dc.journal.title
Sensors and Actuators B: Chemical  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0925400518303721  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.snb.2018.02.094