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Artículo

Cell autoaggregation, biofilm formation, and plant attachment in a sinorhizobium meliloti lpsB mutant

Sorroche, Fernando GuidoIcon ; Bogino, Pablo CesarIcon ; Russo, Daniela MartaIcon ; Zorreguieta, ÁngelesIcon ; Nievas, Fiorela LujanIcon ; Morales, Gustavo Marcel; Hirsch, Ann M.; Giordano, Walter FabianIcon
Fecha de publicación: 10/2018
Editorial: American Phytopathological Society
Revista: Molecular Plant-Microbe Interactions
ISSN: 0894-0282
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Bacterial surface molecules are crucial for the establishment of a successful rhizobia-legume symbiosis, and, in most bacteria, are also critical for adherence properties, surface colonization, and as a barrier for defense. Rhizobial mutants defective in the production of exopolysaccharides (EPSs), lipopolysaccharides (LPSs), or capsular polysaccharides are usually affected in symbiosis with their plant hosts. In the present study, we evaluated the role of the combined effects of LPS and EPS II in cell-to-cell and cell-to-surface interactions in Sinorhizobium meliloti by studying planktonic cell autoaggregation, biofilm formation, and symbiosis with the host plant Medicago sativa. The lpsB mutant, which has a defective core portion of LPS, exhibited a reduction in biofilm formation on abiotic surfaces as well as altered biofilm architecture compared with the wild-type Rm8530 strain. Atomic force microscopy and confocal laser microscopy revealed an increase in polar cell-to-cell interactions in the lpsB mutant, which might account for the biofilm deficiency. However, a certain level of biofilm development was observed in the lpsB strain compared with the EPS II-defective mutant strains. Autoaggregation experiments carried out with LPS and EPS mutant strains showed that both polysaccharides have an impact on the cell-to-cell adhesive interactions of planktonic bacteria. Although the lpsB mutation and the loss of EPS II production strongly stimulated early attachment to alfalfa roots, the number of nodules induced in M. sativa was not increased. Taken together, this work demonstrates that S. meliloti interactions with biotic and abiotic surfaces depend on the interplay between LPS and EPS II.
Palabras clave: Cell Autoaggregation , Biofilm , Plant Attachment
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/92257
URL: https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-18-0004-R
DOI: http://dx.doi.org/10.1094/MPMI-01-18-0004-R
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Sorroche, Fernando Guido; Bogino, Pablo Cesar; Russo, Daniela Marta; Zorreguieta, Ángeles; Nievas, Fiorela Lujan; et al.; Cell autoaggregation, biofilm formation, and plant attachment in a sinorhizobium meliloti lpsB mutant; American Phytopathological Society; Molecular Plant-Microbe Interactions; 31; 10; 10-2018; 1075-1082
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