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dc.contributor.author
Gimenez, Gabriela Gregolin
dc.contributor.author
Costa, Hernán
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de Lima Neto, Quirino Alves
dc.contributor.author
Fernandez, Maria Aparecida
dc.contributor.author
Ferrarotti, Susana Alicia
dc.contributor.author
Matioli, Graciette
dc.date.available
2019-12-13T19:44:05Z
dc.date.issued
2019-04
dc.identifier.citation
Gimenez, Gabriela Gregolin; Costa, Hernán; de Lima Neto, Quirino Alves; Fernandez, Maria Aparecida; Ferrarotti, Susana Alicia; et al.; Sequencing, cloning, and heterologous expression of cyclomaltodextrin glucanotransferase of Bacillus firmus strain 37 in Bacillus subtilis WB800; Springer; Bioprocess And Biosystems Engineering; 42; 4; 4-2019; 621-629
dc.identifier.issn
1615-7591
dc.identifier.uri
http://hdl.handle.net/11336/92220
dc.description.abstract
Bacillusfirmus strain 37 produces the cyclomaltodextrin glucanotransferase (CGTase) enzyme and CGTase produces cyclodextrins (CDs) through a starch cyclization reaction. The strategy for the cloning and expression of recombinant CGTase is a potentially viable alternative for the economically viable production of CGTase for use in industrial processes. The present study used Bacillus subtilis WB800 as a bacterial expression host for the production of recombinant CGTase cloned from the CGTase gene of B. firmus strain 37. The CGTase gene was cloned in TOPO-TA ® plasmid, which was transformed in Escherichia coli DH5α. The subcloning was carried out with pWB980 plasmid and transformation in B. subtilis WB800. The 2xYT medium was the most suitable for the production of recombinant CGTase. The enzymatic activity of the crude extract of the recombinant CGTase of B. subtilis WB800 was 1.33 µmol β-CD/min/mL, or 7.4 times greater than the enzymatic activity of the crude extract of CGTase obtained from the wild strain. Following purification, the recombinant CGTase exhibited an enzymatic activity of 157.78 µmol β-CD/min/mL, while the activity of the CGTase from the wild strain was 9.54 µmol β-CD/min/mL. When optimal CDs production conditions for the CGTase from B. firmus strain 37 were used, it was observed that the catalytic properties of the CGTase enzymes were equivalent. The strategy for the cloning and expression of CGTase in B. subtilis WB800 was efficient, with the production of greater quantities of CGTase than with the wild strain, offering essential data for the large-scale production of the recombinant enzyme.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BACILLUS SUBTILIS WB800
dc.subject
CGTASE
dc.subject
CLONING
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CYCLODEXTRINS
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HETEROLOGOUS EXPRESSION
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Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
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Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Sequencing, cloning, and heterologous expression of cyclomaltodextrin glucanotransferase of Bacillus firmus strain 37 in Bacillus subtilis WB800
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-17T15:36:16Z
dc.identifier.eissn
1615-7605
dc.journal.volume
42
dc.journal.number
4
dc.journal.pagination
621-629
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Gimenez, Gabriela Gregolin. Universidad Estadual de Maringá; Brasil
dc.description.fil
Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
dc.description.fil
Fil: de Lima Neto, Quirino Alves. Universidad Estadual de Maringá; Brasil
dc.description.fil
Fil: Fernandez, Maria Aparecida. Universidad Estadual de Maringá; Brasil
dc.description.fil
Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
dc.description.fil
Fil: Matioli, Graciette. Universidad Estadual de Maringá; Brasil
dc.journal.title
Bioprocess And Biosystems Engineering
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00449-018-02068-4
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00449-018-02068-4
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