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dc.contributor.author
Rojas, Robert L.G.
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Gomes, Priscila A.D.P.
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Bentancor, Leticia Verónica
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Sbrogio Almeida, Maria E.
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Costa, Sérgio O. P.
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Massis, Liliana M.
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Ferreira, Rita C. C.
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Palermo, Marina Sandra
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Ferreira, Luís C. S.
dc.date.available
2019-12-13T19:39:44Z
dc.date.issued
2010-04
dc.identifier.citation
Rojas, Robert L.G.; Gomes, Priscila A.D.P.; Bentancor, Leticia Verónica; Sbrogio Almeida, Maria E.; Costa, Sérgio O. P.; et al.; Salmonella enterica serovar typhimurium vaccine strains expressing a nontoxic shiga-like toxin 2 derivative induce partial protective immunity to the toxin expressed by enterohemorrhagic escherichia coli; American Society for Microbiology; Clinical and Vaccine Immunology; 17; 4; 4-2010; 529-536
dc.identifier.issn
1556-6811
dc.identifier.uri
http://hdl.handle.net/11336/92213
dc.description.abstract
Shiga-like toxin 2 (Stx2)-producing enterohemorrhagic Escherichia coli (referred to as EHEC or STEC) strains are the primary etiologic agents of hemolytic-uremic syndrome (HUS), which leads to renal failure and high mortality rates. Expression of Stx2 is the most relevant virulence-associated factor of EHEC strains, and toxin neutralization by antigen-specific serum antibodies represents the main target for both preventive and therapeutic anti-HUS approaches. In the present report, we describe two Salmonella enterica serovar Typhimurium aroA vaccine strains expressing a nontoxic plasmid-encoded derivative of Stx2 (Stx2δAB) containing the complete nontoxic A2 subunit and the receptor binding B subunit. The two S. Typhimurium strains differ in the expression of flagellin, the structural subunit of the flagellar shaft, which exerts strong adjuvant effects. The vaccine strains expressed Stx2δAB, either cell bound or secreted into the extracellular environment, and showed enhanced mouse gut colonization and high plasmid stability under both in vitro and in vivo conditions. Oral immunization of mice with three doses of the S. Typhimurium vaccine strains elicited serum anti-Stx2B (IgG) antibodies that neutralized the toxic effects of the native toxin under in vitro conditions (Vero cells) and conferred partial protection under in vivo conditions. No significant differences with respect to gut colonization or the induction of antigen-specific antibody responses were detected in mice vaccinated with flagellated versus nonflagellated bacterial strains. The present results indicate that expression of Stx2δAB by attenuated S. Typhimurium strains is an alternative vaccine approach for HUS control, but additional improvements in the immunogenicity of Stx2 toxoids are still required.
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application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
EHEC
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HUS,
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Stx,
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S. Typhimurium;vaccines, flagellin
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Inmunología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Salmonella enterica serovar typhimurium vaccine strains expressing a nontoxic shiga-like toxin 2 derivative induce partial protective immunity to the toxin expressed by enterohemorrhagic escherichia coli
dc.type
info:eu-repo/semantics/article
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info:ar-repo/semantics/artículo
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info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-10T19:32:13Z
dc.journal.volume
17
dc.journal.number
4
dc.journal.pagination
529-536
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington
dc.description.fil
Fil: Rojas, Robert L.G.. Universidade de Sao Paulo; Brasil
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Fil: Gomes, Priscila A.D.P.. Universidade de Sao Paulo; Brasil
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Fil: Bentancor, Leticia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
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Fil: Sbrogio Almeida, Maria E.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
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Fil: Costa, Sérgio O. P.. Universidade de Sao Paulo; Brasil
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Fil: Massis, Liliana M.. Universidade de Sao Paulo; Brasil
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Fil: Ferreira, Rita C. C.. Universidade de Sao Paulo; Brasil
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Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
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Fil: Ferreira, Luís C. S.. Universidade de Sao Paulo; Brasil
dc.journal.title
Clinical and Vaccine Immunology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/CVI.00495-09
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://cvi.asm.org/content/17/4/529
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