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dc.contributor.author
Mirazo Villar, Santiago
dc.contributor.author
Gardinali, Noemí R.
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Cecilia, D'Albora
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Verger, Lorenzo
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Ottonelli, Florencia
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Ramos, Natalia
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Castro, Gustavo
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Pinto, Marcelo A.
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Ré, Viviana Elizabeth
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Pisano, María Belén
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Lozano, Alejandra
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de Oliveira, Jaqueline Mendes
dc.contributor.author
Arbiza, Juan
dc.date.available
2019-12-12T17:41:15Z
dc.date.issued
2018-01
dc.identifier.citation
Mirazo Villar, Santiago; Gardinali, Noemí R.; Cecilia, D'Albora; Verger, Lorenzo; Ottonelli, Florencia; et al.; Serological and virological survey of hepatitis E virus (HEV) in animal reservoirs from Uruguay reveals elevated prevalences and a very close phylogenetic relationship between swine and human strains; Elsevier Science; Veterinary Microbiology; 213; 1-2018; 21-27
dc.identifier.issn
0378-1135
dc.identifier.uri
http://hdl.handle.net/11336/92058
dc.description.abstract
Hepatitis E virus (HEV) infection is an issue of public health concern in high-income and non-endemic countries. Increasing evidence supports the hypothesis of a zoonotic route as the main mode of infection in this epidemiological setting, since the transmission of genotypes HEV-3 and HEV-4 from reservoirs to humans has been demonstrated. In America, studies have confirmed the circulation of HEV in pig herds but the zoonotic role of wild boars has never been evaluated. Uruguay has a high burden of HEV- associated acute hepatitis, and a close phylogenetic relationship was observed among human HEV-3 strains and European isolates detected in swine. However in this context, swine herds have never been surveyed. Herein is reported a survey of HEV in swine herds, pigs at slaughter-house and free-living wild boar populations. Two-hundred and twenty sera and 150 liver tissue samples from domestic pigs, and 140 sera from wild boars were tested for HEV by ELISA and PCR-based approaches. All tested swine farms resulted seropositive with an overall rate of 46.8%. In turn, 22.1% of the wild boars had anti-HEV antibodies. HEV RNA was detected in 16.6% and 9.3% of liver samples from slaughter-age pigs and adult wild boars sera, respectively. Three strains from domestic pig were also amplified by nested-PCR approaches. By contrast, none of the positive samples obtained from wild boars could be confirmed by nested-PCR. Phylogenetic analysis revealed a very high nucleotide identity among swine strains and sequences obtained from humans in Uruguay. Results showed that HEV is widely distributed among swine herds in Uruguay. Additionally, this study evidences for the first time in the American continent that wild boar populations are a reservoir for HEV, though its zoonotic role remains to be elucidated. Altogether, data presented here suggest a high zoonotic risk of HEV transmission from swine to humans.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DOMESTIC PIG
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HEPATITIS E VIRUS
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SURVEY
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WILD BOARS
dc.subject.classification
Enfermedades Infecciosas
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Serological and virological survey of hepatitis E virus (HEV) in animal reservoirs from Uruguay reveals elevated prevalences and a very close phylogenetic relationship between swine and human strains
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-22T17:54:48Z
dc.journal.volume
213
dc.journal.pagination
21-27
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Mirazo Villar, Santiago. Universidad de la República; Uruguay
dc.description.fil
Fil: Gardinali, Noemí R.. Instituto Oswaldo Cruz; Brasil
dc.description.fil
Fil: Cecilia, D'Albora. Universidad de la República; Uruguay
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Fil: Verger, Lorenzo. Universidad de la República; Uruguay
dc.description.fil
Fil: Ottonelli, Florencia. Instituto Oswaldo Cruz; Brasil
dc.description.fil
Fil: Ramos, Natalia. Instituto Oswaldo Cruz; Brasil
dc.description.fil
Fil: Castro, Gustavo. Universidad de la República; Uruguay
dc.description.fil
Fil: Pinto, Marcelo A.. Instituto Oswaldo Cruz; Brasil. Universidad de la República; Uruguay
dc.description.fil
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Pisano, María Belén. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Lozano, Alejandra. Universidad de la República; Uruguay
dc.description.fil
Fil: de Oliveira, Jaqueline Mendes. Instituto Oswaldo Cruz; Brasil
dc.description.fil
Fil: Arbiza, Juan. Universidad de la República; Uruguay
dc.journal.title
Veterinary Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vetmic.2017.11.013
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378113517310957
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