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Artículo

The ERα membrane pool modulates the proliferation of pituitary tumours

Sosa, Liliana del ValleIcon ; Petiti, Juan PabloIcon ; Picech, FlorenciaIcon ; Chumpen Ramirez, Sabrina VanesaIcon ; Nicola, Juan PabloIcon ; Pérez, Pablo AníbalIcon ; de Paul, Ana LuciaIcon ; Valdez Taubas, Javier; Guitierrez, Silvina; Torres, Alicia InesIcon
Fecha de publicación: 02/2019
Editorial: BioScientifica
Revista: Journal of Endocrinology
ISSN: 0022-0795
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Medicina Básica

Resumen

The molecular mechanisms underlying the ERα nuclear/cytoplasmic pool that modulates pituitary cell proliferation have been widely described, but it is still not clear how ERα is targeted to the plasma membrane. The aim of this study was to analyse ERα palmitoylation and the plasma membrane ERα (mERα) pool, and their participation in E2-triggered membrane-initiated signalling in normal and pituitary tumour cell growth. Cell cultures were prepared from anterior pituitaries of female Wistar rats and tumour GH3 cells, and treated with 10 nM of oestradiol (E2). The basal expression of ERα was higher in tumour GH3 than in normal pituitary cells. Full-length palmitoylated ERα was observed in normal and pituitary tumour cells, demonstrating that E2 stimulation increased both, ERα in plasma membrane and ERα and caveolin-1 interaction after short-term treatment. In addition, the Dhhc7 and Dhhc21 palmitoylases were negatively regulated after sustained stimulation of E2 for 3 h. Although the uptake of BrdU into the nucleus in normal pituitary cells was not modified by E2, a significant increase in the GH3 tumoural cell, as well as ERK1/2 activation, with this effect being mimicked by PPT, a selective antagonist of ERα. These proliferative effects were blocked by ICI 182780 and the global inhibitor of palmitoylation. These findings indicate that ERα palmitoylation modulated the mERα pool and consequently the ERK1/2 pathway, thereby contributing to pituitary tumour cell proliferation. These results suggest that the plasma membrane ERα pool might be related to the proliferative behaviour of prolactinoma and may be a marker of pituitary tumour growth.
Palabras clave: OESTROGEN RECEPTOR ALPHA , PALMITOYLATION , PITUITARY TUMOUR , PROLIFERATION
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/91562
DOI: https://doi.org/10.1530/JOE-18-0418
URL: https://joe.bioscientifica.com/view/journals/joe/240/2/JOE-18-0418.xml
Colecciones
Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos(INICSA)
Articulos de INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Citación
Sosa, Liliana del Valle; Petiti, Juan Pablo; Picech, Florencia; Chumpen Ramirez, Sabrina Vanesa; Nicola, Juan Pablo; et al.; The ERα membrane pool modulates the proliferation of pituitary tumours; BioScientifica; Journal of Endocrinology; 240; 2; 2-2019; 229-241
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