Mostrar el registro sencillo del ítem

dc.contributor.author
Sánchez Villalba, Esther  
dc.contributor.author
Arias, María Elena  
dc.contributor.author
Loren, Pía  
dc.contributor.author
Fuentes, Fernanda  
dc.contributor.author
Pereyra Bonnet, Federico Alberto  
dc.contributor.author
Salamone, Daniel Felipe  
dc.contributor.author
Felmer Dörner, Ricardo Nicolás  
dc.date.available
2019-12-04T19:59:58Z  
dc.date.issued
2018-09  
dc.identifier.citation
Sánchez Villalba, Esther; Arias, María Elena; Loren, Pía; Fuentes, Fernanda; Pereyra Bonnet, Federico Alberto; et al.; Improved expression of green fluorescent protein in cattle embryos produced by ICSI-mediated gene transfer with spermatozoa treated with streptolysin-O; Elsevier Science; Animal Reproduction Science; 196; 9-2018; 130-137  
dc.identifier.issn
0378-4320  
dc.identifier.uri
http://hdl.handle.net/11336/91412  
dc.description.abstract
The ICSI-sperm mediated gene transfer (ICSI-SMGT) has been used to produce transgenic mice with high efficiency; however, the efficiency of this technique in farm animals is still less than desirable. Pretreatment of sperm with membrane destabilizing agents can improve the efficiency of ICSI in cattle. The objective of the present study was to evaluate streptolysin-O (SLO) as a novel treatment to permeabilize the bovine sperm membrane and assess its effect on efficiency of generating transgenic embryos by ICSI-SMGT. First, there was evaluation of the plasma membrane integrity (SYBR/PI), acrosome membrane integrity (PNA/FITC), DNA damage (TUNEL) and binding capacity of exogenous DNA (Nick Translation) in bull sperm treated with SLO. Subsequently, there was assessment of embryonic development and the efficiency in generating transgenic embryos with enhanced expression of the gene for green fluorescent protein (EGFP). Results indicate that SLO efficiently permeabilizes the plasma and acrosome membranes of bull spermatozoa and increases binding of exogenous DNA mostly to the post-acrosomal region and tail without greatly affecting the integrity of the DNA. Furthermore, treatment of bull spermatozoa with SLO prior to the injection of oocytes by ICSI-SMGT significantly increased the rate of embryo expression of the EGFP gene. Future experiments are still needed to determine the effect of this treatment on the development and transgene expression in fetuses and animals produced by ICSI-SMGT.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
SLO  
dc.subject
SMGT  
dc.subject
GENE EDITING  
dc.subject
ICSI  
dc.subject.classification
Tecnología GM, clonación de ganado, selección asistida, diagnósticos, tecnología de producción de biomasa, etc.  
dc.subject.classification
Biotecnología Agropecuaria  
dc.subject.classification
CIENCIAS AGRÍCOLAS  
dc.title
Improved expression of green fluorescent protein in cattle embryos produced by ICSI-mediated gene transfer with spermatozoa treated with streptolysin-O  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-10-18T19:07:41Z  
dc.journal.volume
196  
dc.journal.pagination
130-137  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Sánchez Villalba, Esther. Universidad de La Frontera; Chile  
dc.description.fil
Fil: Arias, María Elena. Universidad de La Frontera; Chile  
dc.description.fil
Fil: Loren, Pía. Universidad de La Frontera; Chile  
dc.description.fil
Fil: Fuentes, Fernanda. Universidad de La Frontera; Chile  
dc.description.fil
Fil: Pereyra Bonnet, Federico Alberto. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Felmer Dörner, Ricardo Nicolás. Universidad de La Frontera; Chile  
dc.journal.title
Animal Reproduction Science  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.anireprosci.2018.07.005  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378432018304184