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dc.contributor.author
Vaghi Medina, Carlos Gastón
dc.contributor.author
Teppa, Roxana Elin
dc.contributor.author
Bornancini, Verónica Andrea
dc.contributor.author
Flores, Ceferino René
dc.contributor.author
Marino, Cristina Ester
dc.contributor.author
López Lambertini, Paola María
dc.date.available
2019-12-02T21:00:49Z
dc.date.issued
2018-01
dc.identifier.citation
Vaghi Medina, Carlos Gastón; Teppa, Roxana Elin; Bornancini, Verónica Andrea; Flores, Ceferino René; Marino, Cristina Ester; et al.; Tomato apical leaf curl virus: A novel, monopartite geminivirus detected in tomatoes in Argentina; Frontiers Research Foundation; Frontiers in Microbiology; 8; JAN; 1-2018; 1-11
dc.identifier.issn
1664-302X
dc.identifier.uri
http://hdl.handle.net/11336/91133
dc.description.abstract
Plant viruses that are members of the Geminiviridae family have circular single-stranded DNA (ssDNA) genome and are responsible for major crop diseases worldwide. We have identified and characterized a novel monopartite geminivirus infecting tomato in Argentina. The full-length genome was cloned and sequenced. The genome-wide pairwise identity calculation that resulted in a maximum of 63% identity with all of other known geminiviruses indicated that it is a new geminivirus species. Biolistic infected plants presented interveinal yellowing, apical leaf curling and extreme root hypotrophy. Thus, the name proposed for this species is tomato apical leaf curl virus (ToALCV). The phylogenetic inferences suggested different evolutionary relationships for the replication-associated protein (Rep) and the coat protein (CP). Besides, the sequence similarity network (SSN) protein analyses showed that the complementary-sense gene products (RepA, Rep and C3) are similar to capulavirus while the viron-sense gene products (CP, MP and V3) are similar to topocuvirus, curtovirus and becurtovirus. Based on the data presented, ToALCV genome appears to have "modular organization" supported by its recombination origin. Analyses of the specificity-determining positions (SDPs) of the CP of geminiviruses defined nine subgroups that include geminiviruses that share the same type of insect vector. Our sequences were clustered with the sequences of topocuvirus, whose vector is the treehopper, Micrutalis malleifera. Also, a set of the highest scored amino acid residues was predicted for the CP, which could determine differences in virus transmission specificity. We predict that a treehopper could be the vector of ToALCV, but transmission assays need to be performed to confirm this. Given everything we demonstrate in this paper, ToALCV can be considered a type member of a new putative genus of the Geminiviridae family.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Research Foundation
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ARGENTINA
dc.subject
COAT PROTEIN
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RECOMBINATION
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SEQUENCE SIMILARITY NETWORK
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SPECIFICITY-DETERMINING POSITIONS
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TOALCV
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TOMATO
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TREEHOPPER
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Virología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Tomato apical leaf curl virus: A novel, monopartite geminivirus detected in tomatoes in Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-22T17:45:24Z
dc.journal.volume
8
dc.journal.number
JAN
dc.journal.pagination
1-11
dc.journal.pais
Suiza
dc.description.fil
Fil: Vaghi Medina, Carlos Gastón. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.description.fil
Fil: Teppa, Roxana Elin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil
Fil: Bornancini, Verónica Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Flores, Ceferino René. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil
Fil: Marino, Cristina Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil
Fil: López Lambertini, Paola María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.journal.title
Frontiers in Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fmicb.2017.02665
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2017.02665/full
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