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dc.contributor.author
Sanchez Hernandez, Juan C.
dc.contributor.author
Rios, Juan Manuel
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dc.contributor.author
Attademo, Andres Maximiliano
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dc.date.available
2019-11-29T19:08:55Z
dc.date.issued
2018-09
dc.identifier.citation
Sanchez Hernandez, Juan C.; Rios, Juan Manuel; Attademo, Andres Maximiliano; Response of digestive enzymes and esterases of ecotoxicological concern in earthworms exposed to chlorpyrifos-treated soils; Springer; Ecotoxicology; 27; 7; 9-2018; 890-899
dc.identifier.issn
0963-9292
dc.identifier.uri
http://hdl.handle.net/11336/90987
dc.description.abstract
Assessment of organophosphorus (OP) pesticide exposure in non-target organisms rarely involves non-neural molecular targets. Here we performed a 30-d microcosm experiment with Lumbricus terrestris to determine whether the activity ofdigestive enzymes (phosphatase, β-glucosidase, carboxylesterase and lipase) was sensitive to chlorpyrifos (5 mg kg−1 wet soil). Likewise, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities were measured in the wall muscle and gastrointestinal tissues as indicators of OP exposure. Chlorpyrifos inhibited the acid phosphatase (34% of controls),carboxylesterase (25.6%) and lipase activities (31%) in the gastrointestinal content. However, in the gastrointestinal tissue,only the carboxylesterase and lipase activities were significantly depressed (42?67% carboxylesterase inhibition in the foregut and crop/gizzard, and 15% lipase inhibition in the foregut). Chlorpyrifos inhibited the activity of both cholinesterasesin the gastrointestinal tissues, whereas the AChE activity was affected in the wall muscle. These results suggested chlorpyrifos was widely distributed throughout the earthworm body after 30 d of incubation. Interestingly, we found muscle carboxylesterase activity strongly inhibited (92% of control) compared with that detected in the gastrointestinal tissues of the same OP-exposed individuals. This finding was explained by the occurrence of pesticide-resistant esterases in the gastrointestinal tissues, which were evidenced by zymography. Our results suggest that digestive processes of L. terrestris may be altered by chlorpyrifos, as a consequence of the inhibitory action of the insecticide on some digestive enzymes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Lumbricus terrestris
dc.subject
Digestive enzymes
dc.subject
Cholinesterase
dc.subject
Zymography
dc.subject
Carboxylesterases
dc.subject
Lipases
dc.subject.classification
Otras Ciencias de la Tierra y relacionadas con el Medio Ambiente
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dc.subject.classification
Ciencias de la Tierra y relacionadas con el Medio Ambiente
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Response of digestive enzymes and esterases of ecotoxicological concern in earthworms exposed to chlorpyrifos-treated soils
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-28T16:46:58Z
dc.journal.volume
27
dc.journal.number
7
dc.journal.pagination
890-899
dc.journal.pais
Alemania
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dc.journal.ciudad
Berlin
dc.description.fil
Fil: Sanchez Hernandez, Juan C.. Universidad de Castilla-La Mancha; España
dc.description.fil
Fil: Rios, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
dc.description.fil
Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
dc.journal.title
Ecotoxicology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s10646-018-1914-8
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