Artículo
Mucin and carbon nanotube-based biosensor for detection of glucose in human plasma
Fecha de publicación:
06/2018
Editorial:
Academic Press Inc Elsevier Science
Revista:
Analytical Biochemistry
ISSN:
0003-2697
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
This work reports an amperometric enzyme-electrode prepared with glucose oxidase, which have been immobilized by a cross-linking step with glutaraldehyde in a mixture containing albumin and a novel carbon nanotubes-mucin composite (CNT-muc). The obtained hydrogel matrix was trapped between two polycarbonate membranes and then fixed at the surface of a Pt working electrode. The developed biosensor was optimized by evaluating different compositions and the analytical properties of an enzymatic matrix with CNT-muc. Then, the performance of the resulting enzymatic matrix was evaluated for direct glucose quantification in human blood plasma. The novel CNT-muc composite provided a sensitivity of 0.44 ± 0.01 mA M −1 and a response time of 28 ± 2 s. These values were respectively 20% higher and 40% shorter than those obtained with a sandwich-type biosensor prepared without CNT. Additionally, CNT-muc based biosensor exhibited more than 3 orders of magnitude of linear dynamic calibration range and a detection limit of 3 μM. The short-term and long-term stabilities of the biosensors were also examined and excellent results were obtained through successive experiments performed within the first 60 days from their preparation. Finally, the storage stability was remarkable during the first 300 days.
Palabras clave:
BIOSENSOR
,
CARBON NANOTUBES
,
GLUCOSE OXIDASE
,
HUMAN PLASMA
,
MUCIN
,
NOVEL HYDROGEL
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Identificadores
Colecciones
Articulos(INFIQC)
Articulos de INST.DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Articulos de INST.DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Articulos(IPQA)
Articulos deINSTITUTO DE INVESTIGACION Y DESARROLLO EN INGENIERIA DE PROCESOS Y QUIMICA APLICADA
Articulos deINSTITUTO DE INVESTIGACION Y DESARROLLO EN INGENIERIA DE PROCESOS Y QUIMICA APLICADA
Citación
Comba, Fausto Nahuel; Romero, Marcelo Ricardo; Garay, Fernando Sebastian; Baruzzi, Ana Maria; Mucin and carbon nanotube-based biosensor for detection of glucose in human plasma; Academic Press Inc Elsevier Science; Analytical Biochemistry; 550; 6-2018; 34-40
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