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dc.contributor.author
Edelsztein, Nadia Yasmín  
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Racine, Chrystèle  
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di Clemente, Nathalie  
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Schteingart, Helena Fedora  
dc.contributor.author
Rey, Rodolfo Alberto  
dc.date.available
2019-11-28T22:46:30Z  
dc.date.issued
2018-12  
dc.identifier.citation
Edelsztein, Nadia Yasmín; Racine, Chrystèle; di Clemente, Nathalie; Schteingart, Helena Fedora; Rey, Rodolfo Alberto; Androgens downregulate anti-Müllerian hormone promoter activity in the Sertoli cell through the androgen receptor and intact steroidogenic factor 1 sites; Society for the Study of Reproduction; Biology of Reproduction; 99; 6; 12-2018; 1303-1312  
dc.identifier.issn
0006-3363  
dc.identifier.uri
http://hdl.handle.net/11336/90864  
dc.description.abstract
Testicular anti-Müllerian hormone (AMH) production is inhibited by androgens around pubertal onset, as observed under normal physiological conditions and in patients with precocious puberty. In agreement, AMH downregulation is absent in patients with androgen insensitivity. The molecular mechanisms underlying the negative regulation of AMH by androgens remain unknown. Our aim was to elucidate the mechanisms through which androgens downregulate AMH expression in the testis. A direct negative effect of androgens on the transcriptional activity of the AMH promoter was found using luciferase reporter assays in the mouse prepubertal Sertoli cell line SMAT1. A strong inhibition of AMH promoter activity was seen in the presence of both testosterone and DHT and of the androgen receptor. By site-directed mutagenesis and chromatin immunoprecipitation assays, we showed that androgen-mediated inhibition involved the binding sites for steroidogenic factor 1 (SF1) present in the proximal promoter of the AMH gene. In this study, we describe for the first time the mechanism behind AMH inhibition by androgens, as seen in physiological and pathological conditions in males. Inhibition of AMH promoter activity by androgens could be due to protein-protein interactions between the ligand-bound androgen receptor and SF1 or by blockage of SF1 binding to its sites on the AMH promoter.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Society for the Study of Reproduction  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
INHIBITION  
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PUBERTY  
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STEROID HORMONES/STEROID HORMONE RECEPTORS  
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TESTIS  
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TESTOSTERONE  
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TRANSCRIPTIONAL REGULATION  
dc.subject.classification
Fisiología  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Androgens downregulate anti-Müllerian hormone promoter activity in the Sertoli cell through the androgen receptor and intact steroidogenic factor 1 sites  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-10-16T15:08:06Z  
dc.journal.volume
99  
dc.journal.number
6  
dc.journal.pagination
1303-1312  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Madison  
dc.description.fil
Fil: Edelsztein, Nadia Yasmín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina  
dc.description.fil
Fil: Racine, Chrystèle. Inserm; Francia  
dc.description.fil
Fil: di Clemente, Nathalie. Inserm; Francia  
dc.description.fil
Fil: Schteingart, Helena Fedora. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina  
dc.description.fil
Fil: Rey, Rodolfo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina  
dc.journal.title
Biology of Reproduction  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/biolre/ioy152  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article-abstract/99/6/1303/5050366