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Artículo

The disruptive effect of lysozyme on the bacterial cell wall explored by an in-silico structural outlook

Primo, Emiliano DavidIcon ; Otero, Lisandro HoracioIcon ; Ruiz, Francisco OscarIcon ; Klinke, SebastianIcon ; Giordano, Walter FabianIcon
Fecha de publicación: 01/2018
Editorial: John Wiley & Sons Inc
Revista: Biochemistry and Molecular Biology Education
ISSN: 1470-8175
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

The bacterial cell wall, a structural unit of peptidoglycan polymer comprised of glycan strands consisting of a repeating disaccharide motif [N-acetylglucosamine (NAG) and N-acetylmuramylpentapeptide (NAM pentapeptide)], encases bacteria and provides structural integrity and protection. Lysozymes are enzymes that break down the bacterial cell wall and disrupt the bacterial life cycle by cleaving the linkage between the NAG and NAM carbohydrates. Lab exercises focused on the effects of lysozyme on the bacterial cell wall are frequently incorporated in biochemistry classes designed for undergraduate students in diverse fields as biology, microbiology, chemistry, agronomy, medicine, and veterinary medicine. Such exercises typically do not include structural data. We describe here a sequence of computer tasks designed to illustrate and reinforce both physiological and structural concepts involved in lysozyme effects on the bacterial cell-wall structure. This lab class usually lasts 3.5 hours. First, the instructor presents introductory concepts of the bacterial cell wall and the effect of lysozyme on its structure. Then, students are taught to use computer modeling to visualize the three-dimensional structure of a lysozyme in complex with bacterial cell-wall fragments. Finally, the lysozyme inhibitory effect on a bacterial culture is optionally proposed as a simple microbiological assay. The computer lab exercises described here give students a realistic understanding of the disruptive effect of lysozymes on the bacterial cell wall, a crucial component in bacterial survival.
Palabras clave: BACTERIAL CELL WALL , LYSOZYME , PROTEIN DATA BANK SERVER , PYMOL PROGRAM
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/90845
DOI: http://dx.doi.org/10.1002/bmb.21092
URL: https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/bmb.21092
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Primo, Emiliano David; Otero, Lisandro Horacio; Ruiz, Francisco Oscar; Klinke, Sebastian; Giordano, Walter Fabian; The disruptive effect of lysozyme on the bacterial cell wall explored by an in-silico structural outlook; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 46; 1; 1-2018; 83-90
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