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Artículo

Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration

Unsain, NicolasIcon ; Bordenave, Martín DiegoIcon ; Martinez, Gaby F.; Jalil, Sami; Von Bilderling, CatalinaIcon ; Barabas, Federico MartínIcon ; Masullo, Luciano AndrésIcon ; Johnstone, Aaron D.; Barker, Philip A.; Bisbal, MarianoIcon ; Stefani, Fernando DanielIcon ; Caceres, Alfredo OscarIcon
Fecha de publicación: 12/2018
Editorial: Nature Publishing Group
Revista: Scientific Reports
ISSN: 2045-2322
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Axonal degeneration occurs in the developing nervous system for the appropriate establishment of mature circuits, and is also a hallmark of diverse neurodegenerative diseases. Despite recent interest in the field, little is known about the changes (and possible role) of the cytoskeleton during axonal degeneration. We studied the actin cytoskeleton in an in vitro model of developmental pruning induced by trophic factor withdrawal (TFW). We found that F-actin decrease and growth cone collapse (GCC) occur early after TFW; however, treatments that prevent axonal fragmentation failed to prevent GCC, suggesting independent pathways. Using super-resolution (STED) microscopy we found that the axonal actin/spectrin membrane-associated periodic skeleton (MPS) abundance and organization drop shortly after deprivation, remaining low until fragmentation. Fragmented axons lack MPS (while maintaining microtubules) and acute pharmacological treatments that stabilize actin filaments prevent MPS loss and protect from axonal fragmentation, suggesting that MPS destruction is required for axon fragmentation to proceed.
Palabras clave: NANOSCOPY , AXON , DEGENERATION , NEURONS
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/90541
DOI: http://dx.doi.org/10.1038/s41598-018-21232-0
URL: https://www.nature.com/articles/s41598-018-21232-0
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Articulos(CIBION)
Articulos de CENTRO DE INVESTIGACIONES EN BIONANOCIENCIAS "ELIZABETH JARES ERIJMAN"
Citación
Unsain, Nicolas; Bordenave, Martín Diego; Martinez, Gaby F.; Jalil, Sami; Von Bilderling, Catalina; et al.; Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-16
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