Artículo
Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration
Unsain, Nicolas
; Bordenave, Martín Diego
; Martinez, Gaby F.; Jalil, Sami; Von Bilderling, Catalina
; Barabas, Federico Martín
; Masullo, Luciano Andrés
; Johnstone, Aaron D.; Barker, Philip A.; Bisbal, Mariano
; Stefani, Fernando Daniel
; Caceres, Alfredo Oscar
Fecha de publicación:
12/2018
Editorial:
Nature Publishing Group
Revista:
Scientific Reports
ISSN:
2045-2322
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Axonal degeneration occurs in the developing nervous system for the appropriate establishment of mature circuits, and is also a hallmark of diverse neurodegenerative diseases. Despite recent interest in the field, little is known about the changes (and possible role) of the cytoskeleton during axonal degeneration. We studied the actin cytoskeleton in an in vitro model of developmental pruning induced by trophic factor withdrawal (TFW). We found that F-actin decrease and growth cone collapse (GCC) occur early after TFW; however, treatments that prevent axonal fragmentation failed to prevent GCC, suggesting independent pathways. Using super-resolution (STED) microscopy we found that the axonal actin/spectrin membrane-associated periodic skeleton (MPS) abundance and organization drop shortly after deprivation, remaining low until fragmentation. Fragmented axons lack MPS (while maintaining microtubules) and acute pharmacological treatments that stabilize actin filaments prevent MPS loss and protect from axonal fragmentation, suggesting that MPS destruction is required for axon fragmentation to proceed.
Palabras clave:
NANOSCOPY
,
AXON
,
DEGENERATION
,
NEURONS
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Articulos(CIBION)
Articulos de CENTRO DE INVESTIGACIONES EN BIONANOCIENCIAS "ELIZABETH JARES ERIJMAN"
Articulos de CENTRO DE INVESTIGACIONES EN BIONANOCIENCIAS "ELIZABETH JARES ERIJMAN"
Citación
Unsain, Nicolas; Bordenave, Martín Diego; Martinez, Gaby F.; Jalil, Sami; Von Bilderling, Catalina; et al.; Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-16
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