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dc.contributor.author
Huang, Cheng Chiu
dc.contributor.author
Carcagno, Abel Luis
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dc.date.available
2019-11-26T18:08:34Z
dc.date.issued
2018-02
dc.identifier.citation
Huang, Cheng Chiu; Carcagno, Abel Luis; Electroporation of postimplantation mouse embryos in utero; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2018; 2; 2-2018; 99-102
dc.identifier.issn
1940-3402
dc.identifier.uri
http://hdl.handle.net/11336/90524
dc.description.abstract
Gene transfer by electroporation is possible in mouse fetuses within the uterus. As described in this protocol, the pregnant female is anesthetized, the abdominal cavity is opened, and the uterus with the fetuses is exteriorized. A solution of plasmid DNA is injected through the uterine wall directly into the fetus, typically into a cavity like the brain ventricle, guided by fiber optic illumination. Electrodes are positioned on the uterus around the region of the fetus that was injected, and electrical pulses are delivered. The uterus is returned to the abdominal cavity, the body wall is sutured closed, and the female is allowed to recover. The manipulated fetuses can then be collected and analyzed at various times after the electroporation. This method allows experimental access to later-stage developing mouse embryos.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cold Spring Harbor Laboratory Press
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ELECTROPORATION
dc.subject
MOUSE
dc.subject
IN UTERO
dc.subject
EMBRYO
dc.subject.classification
Biología del Desarrollo
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Electroporation of postimplantation mouse embryos in utero
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-22T18:03:02Z
dc.journal.volume
2018
dc.journal.number
2
dc.journal.pagination
99-102
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Huang, Cheng Chiu. University of Texas MD Anderson Cancer Center; Argentina
dc.description.fil
Fil: Carcagno, Abel Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.journal.title
Cold Spring Harbor Protocols
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.cshprotocols.org/lookup/doi/10.1101/pdb.prot094060
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1101/pdb.prot094060
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