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dc.contributor.author
Masullo, Luciano Andrés
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dc.contributor.author
Bodén, Andreas
dc.contributor.author
Pennacchietti, Francesca
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Coceano, Giovanna
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Ratz, Michael
dc.contributor.author
Testa, Ilaria
dc.date.available
2019-11-25T21:32:26Z
dc.date.issued
2018-12
dc.identifier.citation
Masullo, Luciano Andrés; Bodén, Andreas; Pennacchietti, Francesca; Coceano, Giovanna; Ratz, Michael; et al.; Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems; Nature Publishing Group; Nature Communications; 9; 1; 12-2018; 1-9
dc.identifier.issn
2041-1723
dc.identifier.uri
http://hdl.handle.net/11336/90375
dc.description.abstract
The theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging structures at a scale of 45–65 nm within the entire cell volume at low light intensities (W-kW cm−2). Our approach, based on reversibly switchable fluorescent proteins, features three distinctly modulated illumination patterns crafted and combined to gain fluorescence ON–OFF switching cycles and image contrast. By maximizing the detected photon flux, MoNaLISA enables prolonged (40–50 frames) and large (50 × 50 µm2) recordings at 0.3–1.3 Hz with enhanced optical sectioning ability. We demonstrate the general use of our approach by 4D imaging of organelles and fine structures in epithelial human cells, colonies of mouse embryonic stem cells, brain cells, and organotypic tissues.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
NANOSCOPY
dc.subject
LIVING
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CELLS
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TIMELAPSE
dc.subject.classification
Óptica
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dc.subject.classification
Ciencias Físicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-21T13:35:02Z
dc.journal.volume
9
dc.journal.number
1
dc.journal.pagination
1-9
dc.journal.pais
Reino Unido
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dc.description.fil
Fil: Masullo, Luciano Andrés. Royal Institute of Technology; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
dc.description.fil
Fil: Bodén, Andreas. Royal Institute of Technology; Suecia
dc.description.fil
Fil: Pennacchietti, Francesca. Royal Institute of Technology; Suecia
dc.description.fil
Fil: Coceano, Giovanna. Royal Institute of Technology; Suecia
dc.description.fil
Fil: Ratz, Michael. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
dc.description.fil
Fil: Testa, Ilaria. Royal Institute of Technology; Suecia
dc.journal.title
Nature Communications
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/s41467-018-05799-w
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41467-018-05799-w
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