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dc.contributor.author
Gonzalez, Candela Rocio
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dc.contributor.author
Calandra, Ricardo Saul
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dc.contributor.author
Gonzalez Calvar, Silvia I.
dc.date.available
2016-12-07T19:01:31Z
dc.date.issued
2012-12
dc.identifier.citation
Gonzalez, Candela Rocio; Calandra, Ricardo Saul; Gonzalez Calvar, Silvia I.; Influence of the photoperiod on TGF-β1 and p15 expression in hamster Leydig cells; Inst Animal Reproduction Food Research; Reproductive Biology; 12; 2; 12-2012; 201-218
dc.identifier.issn
1642-431X
dc.identifier.uri
http://hdl.handle.net/11336/9009
dc.description.abstract
Adult hamsters exposed to short photoperiods show a marked atrophy of their internal reproductive organs, including a reduction in size, though not number of Leydig cells. Transforming growth factor-β1 (TGF-β1) is involved in the regulation of growth and proliferation of different cell types. The aim of the present study was to examine the influence of photoperiod on the protein and gene expression of TGF-β1 and its receptors as well as gene expression of p15. The effect of TGF-β1 on the expression of p15 in purified Leydig cells from regressed and non-regressed hamster testes was also tested. Protein and gene expression of TGF-β1 was detected in both regressed and non-regressed testes.
In contrast to the activin receptor-like kinase 1 (ALK-1), the TGF-β1, the activin receptor-like kinase 5 (ALK-5) and the co-receptor endoglin all showed a greater basal expression in regressed than non-regressed hamster testes. Melatonin induced the TGF-β1 mRNA expression in purified Leydig cells from non-regressed testes. The p15 mRNA level was greater in regressed than non-regressed testes. A high dose of TGF-β1 during a short incubation period increased the p15 mRNA level in Leydig cells from non-regressed testes. ALK-5 and mitogen-activated protein kinase (MAPK) p38 might have played a role in this process. In regressed hamster testes, the p15 mRNA level increased due to a low dose of TGF-β1 after short incubation periods and to a high dose after longer incubation periods; in both instances, ALK-5, ERK 1/2 and p38 were involved. Collectively, these results suggest that the alterations in p15 expression, mediated by MAPK, are involved in the shift between the active and inactive states in hamster Leydig cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Inst Animal Reproduction Food Research
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Leydig
dc.subject
Tgf-Beta1
dc.subject
P15
dc.subject
Photoperiod
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Influence of the photoperiod on TGF-β1 and p15 expression in hamster Leydig cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-22T21:21:28Z
dc.journal.volume
12
dc.journal.number
2
dc.journal.pagination
201-218
dc.journal.pais
Polonia
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dc.journal.ciudad
Olsztyn
dc.description.fil
Fil: Gonzalez, Candela Rocio. Universidad Maimónides. Area de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Gonzalez Calvar, Silvia I.. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.journal.title
Reproductive Biology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1642431X12600862
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/S1642-431X(12)60086-2
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