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Artículo

The MAP3K-coding QUI-GON JINN (QGJ) gene is essential to the formation of unreduced embryo sacs in paspalum

Mancini, MicaelaIcon ; Permingeat, Hugo Raúl; Colono, Carolina MartaIcon ; Siena, Lorena AdelinaIcon ; Pupilli, Fulvio; Azzaro, Celeste AntonelaIcon ; Alencar Dusi, Diva Maria de; Campos Carneiro, Vera Tavares de; Podio, MaricelIcon ; Seijo, José GuillermoIcon ; González, Ana MaríaIcon ; Felitti, Silvina AndreaIcon ; Ortiz, Juan Pablo AmelioIcon ; Leblanc, Olivier; Pessino, Silvina ClaudiaIcon
Fecha de publicación: 10/2018
Editorial: Frontiers Media S.A.
Revista: Frontiers in Plant Science
ISSN: 1664-462X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Ciencias de las Plantas, Botánica; Genética y Herencia

Resumen

Apomixis is a clonal mode of reproduction via seeds, which results from the failure of meiosis and fertilization in the sexual female reproductive pathway. In previous transcriptomic surveys, we identified a mitogen-activated protein kinase kinase kinase (N46) displaying differential representation in florets of sexual and apomictic Paspalum notatum genotypes. Here, we retrieved and characterized the N46 full cDNA sequence from sexual and apomictic floral transcriptomes. Phylogenetic analyses showed that N46 was a member of the YODA family, which was re-named QUI-GON JINN (QGJ). Differential expression in florets of sexual and apomictic plants was confirmed by qPCR. In situ hybridization experiments revealed expression in the nucellus of aposporous plants’ ovules, which was absent in sexual plants. RNAi inhibition of QGJ expression in two apomictic genotypes resulted in significantly reduced rates of aposporous embryo sac formation, with respect to the level detected in wild type aposporous plants and transformation controls. The QGJ locus segregated independently of apospory. However, a probe derived from a related long non-coding RNA sequence (PN_LNC_QGJ) revealed RFLP bands cosegregating with the Paspalum apospory-controlling region (ACR). PN_LNC_QGJ is expressed in florets of apomictic plants only. Our results indicate that the activity of QGJ in the nucellus of apomictic plants is necessary to form non-reduced embryo sacs and that a long non-coding sequence with regulatory potential is similar to sequences located within the ACR.
Palabras clave: APOMIXIS , APOSPORY , LNC-QGJ , MAP3K , PASPALUM NOTATUM , PLANT REPRODUCTION , QGJ , QUI-GON
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution 2.5 Unported (CC BY 2.5)
Identificadores
URI: http://hdl.handle.net/11336/89615
DOI: https://doi.org/10.3389/fpls.2018.01547
URL: https://www.frontiersin.org/articles/10.3389/fpls.2018.01547
Colecciones
Articulos(IBONE)
Articulos de INST.DE BOTANICA DEL NORDESTE (I)
Articulos(IICAR)
Articulos de INST. DE INVESTIGACIONES EN CIENCIAS AGRARIAS DE ROSARIO
Citación
Mancini, Micaela; Permingeat, Hugo Raúl; Colono, Carolina Marta; Siena, Lorena Adelina; Pupilli, Fulvio; et al.; The MAP3K-coding QUI-GON JINN (QGJ) gene is essential to the formation of unreduced embryo sacs in paspalum; Frontiers Media S.A.; Frontiers in Plant Science; 9; 1547; 10-2018; 1-15
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