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dc.contributor.author
Varmira, Kambiz
dc.contributor.author
Abdi, Omid
dc.contributor.author
Gholivand, Mohammad Bagher
dc.contributor.author
Goicoechea, Hector Casimiro
dc.contributor.author
Jalalvand, Ali R.
dc.date.available
2019-11-21T16:50:51Z
dc.date.issued
2018-01
dc.identifier.citation
Varmira, Kambiz; Abdi, Omid; Gholivand, Mohammad Bagher; Goicoechea, Hector Casimiro; Jalalvand, Ali R.; Intellectual modifying a bare glassy carbon electrode to fabricate a novel and ultrasensitive electrochemical biosensor: Application to determination of acrylamide in food samples; Elsevier Science; Talanta; 176; 1-2018; 509-517
dc.identifier.issn
0039-9140
dc.identifier.uri
http://hdl.handle.net/11336/89417
dc.description.abstract
Acrylamide (AA) is a neurotoxin and carcinogen which is mainly formed in foods containing large quantities of starch processed at high temperatures and its determination is very important to control the quality of foods. In this work, a novel electrochemical biosensor based on hemoglobin-dimethyldioctadecylammonium bromide (HG-DDAB)/platinum-gold-palladium three metallic alloy nanoparticles (PtAuPd NPs)/chitosan-1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (Ch-IL)/multiwalled carbon nanotubes-IL (MWCNTs-IL)/glassy carbon electrode (GCE) is proposed for ultrasensitive determination of AA in food samples. Development of the biosensor is based on forming an adduct by the reaction of AA with α-NH2 group of N-terminal valine of HG which decreases the peak current of HG-Fe+3 reduction. The modifications were characterized by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), energy dispersive X-ray spectroscopic (EDS) and scanning electron microscopy (SEM). Under optimized conditions, the biosensor detected AA by square wave voltammetry (SWV) in two linear concentration ranges of 0.03?39.0 nM and 39.0?150.0 nM with a limit of detection (LOD) of 0.01 nM. The biosensor was able to selective detection of AA even in the presence of high concentrations of common interferents which confirmed that the biosensor is highly selective. Also, the results obtained from further studies confirmed that the proposed biosensor has a short response time (less than 8 s), good sensitivity, long term stability, repeatability, and reproducibility. Finally, the proposed biosensor was successfully applied to determine AA in potato chips and its results were comparable to those obtained by gas chromatography-mass spectrometry (GC-MS) as reference method.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Acrylamide
dc.subject
Hemoglobin
dc.subject
Adduct
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Biosensor
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Square wave voltammetry
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Food samples
dc.subject.classification
Química Analítica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Intellectual modifying a bare glassy carbon electrode to fabricate a novel and ultrasensitive electrochemical biosensor: Application to determination of acrylamide in food samples
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-28T16:52:55Z
dc.journal.volume
176
dc.journal.pagination
509-517
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Varmira, Kambiz. Kermanshah University Of Medical Sciences; Irán
dc.description.fil
Fil: Abdi, Omid. Kermanshah University Of Medical Sciences; Irán
dc.description.fil
Fil: Gholivand, Mohammad Bagher. Razi University; Irán
dc.description.fil
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
dc.description.fil
Fil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; Irán
dc.journal.title
Talanta
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0039914017308974
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.talanta.2017.08.069
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