Artículo
Evaluation of the immunogenicity of a recombinant HSV-1 vector expressing human group C rotavirus VP6 protein
Rota, Rosana Paola
; Palacios, Carlos Adolfo
; Temprana, Carlos Facundo
; Argüelles, Marcelo Horacio
; Mandile, Marcelo Gastón
; Mattion, Nora Marta
; Laimbacher, Andrea S.; Fraefel, Cornell; Castello, Alejandro Andrés; Almallo de Glikmann, Graciela
Fecha de publicación:
06/2018
Editorial:
Elsevier Science
Revista:
Journal of Virological Methods
ISSN:
0166-0934
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Group C Rotavirus (RVC) has been associated globally with sporadic outbreaks of gastroenteritis in children and adults. RVC also infects animals, and interspecies transmission has been reported as well as its zoonotic potential. Considering its genetic diversity and the absence of effective vaccines, it is important and necessary to develop new generation vaccines against RVC for both humans and animals. The aim of the present study was to develop and characterize an HSV-1-based amplicon vector expressing a human RVC-VP6 protein and evaluate the humoral immune response induced after immunizing BALB/c mice. Local fecal samples positive for RVC were used for isolation and sequencing of the vp6 gene, which phylogenetically belongs to the I2 genotype. We show here that cells infected with the HSV[VP6C] amplicon vector efficiently express the VP6 protein, and induced specific anti-RVC antibodies in mice immunized with HSV[VP6C], in a prime-boost schedule. This work highlights that amplicon vectors are an attractive platform for the generation of safe genetic immunogens against RVC, without the addition of external adjuvants.
Palabras clave:
HSV AMPLICON VECTOR
,
ROTAVIRUS
,
VP6
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Colecciones
Articulos(ICT - MILSTEIN)
Articulos de INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN"
Articulos de INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN"
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Rota, Rosana Paola; Palacios, Carlos Adolfo; Temprana, Carlos Facundo; Argüelles, Marcelo Horacio; Mandile, Marcelo Gastón; et al.; Evaluation of the immunogenicity of a recombinant HSV-1 vector expressing human group C rotavirus VP6 protein; Elsevier Science; Journal of Virological Methods; 256; 6-2018; 24-31
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