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dc.contributor.author
Martin, Anandi
dc.contributor.author
Imperiale, Belén Rocío
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Ravolonandriana, Pascaline
dc.contributor.author
Coban, Ahmet Yilmaz
dc.contributor.author
Akgunes, Alper
dc.contributor.author
Ikram, Aamer
dc.contributor.author
Satti, Luqman
dc.contributor.author
Odoun, Mathieu
dc.contributor.author
Pandey, Pooja
dc.contributor.author
Mishra, Manvi
dc.contributor.author
Affolabi, Dissou
dc.contributor.author
Singh, Urvashi
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Rasolofo, Voahangy
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Morcillo, Nora Susana
dc.contributor.author
Vandamme, Peter
dc.contributor.author
Palomino, Juan Carlos
dc.date.available
2019-11-13T21:49:32Z
dc.date.issued
2014-02
dc.identifier.citation
Martin, Anandi; Imperiale, Belén Rocío; Ravolonandriana, Pascaline; Coban, Ahmet Yilmaz; Akgunes, Alper; et al.; Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis; Oxford University Press; Journal of Antimicrobial Chemotherapy; 69; 2; 2-2014; 441-444
dc.identifier.issn
0305-7453
dc.identifier.uri
http://hdl.handle.net/11336/88817
dc.description.abstract
Objectives: To perform a multicentre study evaluating the performance of the direct nitrate reductase assay (NRA) for the detection of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis in sputum samples. Methods: The study was conducted in six laboratories performing tuberculosis diagnosis that were located in six different countries. The NRA was performed directly on sputum samples in parallel with the reference method used at each site. Detection of resistance was performed for rifampicin, isoniazid, ofloxacin and kanamycin. Results: Excellent agreement was obtained for all drugs tested at the majority of sites. The accuracy was 93.7%-100% for rifampicin, 88.2%-100% for isoniazid, 94.6%-100% for ofloxacin and 100% for kanamycin. The majority of NRA results were available at day 21 for sites 1, 2 and 5. Site 3 had a turnaround time of 13.9 days, at site 4 it was 18.4 days and at site 6 it was 16.2 days. The contamination rate ranged between 2.5% and 12%. Conclusions: Rapid detection of drug resistance by the direct NRA on sputum smear-positive samples was accurate and easy to implement in clinical diagnostic laboratories, making it a good alternative for rapid screening for MDR and XDR tuberculosis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
MULTIDRUG RESISTANCE
dc.subject
MYCOBACTERIUM TUBERCULOSIS
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NITRATASE
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-21T15:58:07Z
dc.journal.volume
69
dc.journal.number
2
dc.journal.pagination
441-444
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Martin, Anandi. University of Ghent; Bélgica
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Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
dc.description.fil
Fil: Ravolonandriana, Pascaline. Institut Pasteur de Madagascar; Madagascar
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Fil: Coban, Ahmet Yilmaz. Ondokuz Mayis University; Turquía
dc.description.fil
Fil: Akgunes, Alper. Chest Diseases Hospital; Turquía
dc.description.fil
Fil: Ikram, Aamer. Combined Military Hospital; Pakistán
dc.description.fil
Fil: Satti, Luqman. Combined Military Hospital; Pakistán
dc.description.fil
Fil: Odoun, Mathieu. Laboratoire de Reference des Mycobacterie; Benín
dc.description.fil
Fil: Pandey, Pooja. All India Institute of Medical Sciences; India
dc.description.fil
Fil: Mishra, Manvi. All India Institute of Medical Sciences; India
dc.description.fil
Fil: Affolabi, Dissou. Laboratoire de Reference des Mycobacterie; Benín
dc.description.fil
Fil: Singh, Urvashi. All India Institute of Medical Sciences; India
dc.description.fil
Fil: Rasolofo, Voahangy. Institut Pasteur de Madagascar; Madagascar
dc.description.fil
Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
dc.description.fil
Fil: Vandamme, Peter. University of Ghent; Bélgica
dc.description.fil
Fil: Palomino, Juan Carlos. University of Ghent; Bélgica
dc.journal.title
Journal of Antimicrobial Chemotherapy
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/jac/dkt353
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jac/article/69/2/441/711920


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