Artículo
Kinetics and growth mechanism of the photoinduced synthesis of silver nanoparticles stabilized with lysozyme
Rey, Valentina
; Gramajo Feijoo, Marcelo Eduardo
; Gimenez, Rodrigo Esteban
; Tuttolomondo, María Eugenia; Moran Vieyra, Faustino Eduardo
; Sosa Morales, Marcelo Clemente
; Borsarelli, Claudio Darío
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Fecha de publicación:
12/2018
Editorial:
Elsevier Science
Revista:
Colloids and Surfaces B: Biointerfaces
ISSN:
0927-7765
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A fast and single-step procedure is reported for the preparation of stable solutions of spherical-shaped silver nanoparticles (AgNPs) coated with lysozyme (LZ). The preparation of the AgNP@LZ nanocomposites was based on the reduction of Ag + with ketyl radicals photo-generated by the UVA-photolysis of the benzoin I-2959. Both reaction precursors bind to LZ, modifying its superficial charge and conformational structure. The photo-induced kinetics of formation of the AgNPs as a function of the LZ concentration was monitored in-situ by UV–vis absorption spectroscopy. The multivariate curve resolution-alternating least square (MCR–ALS) method was used for the deconvolution of the kinetic curves for each transient species formed before the growth of the final AgNPs colloids. The Kolmogorov-Johnson-Mehl-Avrami (KJMA) model to describe the formation of the AgNPs was used, and the respective first-order rate constants for the growth of the AgNPs as a function of the lysozyme concentration were calculated and the role of the protein capping in the growth kinetics was evaluated. Despite the protein being partially oxidized by the photo-generated radicals, it was strongly adsorbed onto the silver surface forming a tight coating shell around the AgNPs of approximately 30–60 protein molecules. As a result of the partial denaturation and crowded packing, its intrinsic lytic activity was strongly reduced.
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Colecciones
Articulos(INBIONATEC)
Articulos de INSTITUTO DE BIONANOTECNOLOGIA DEL NOA
Articulos de INSTITUTO DE BIONANOTECNOLOGIA DEL NOA
Articulos(INIFTA)
Articulos de INST.DE INV.FISICOQUIMICAS TEORICAS Y APLIC.
Articulos de INST.DE INV.FISICOQUIMICAS TEORICAS Y APLIC.
Articulos(INQUINOA)
Articulos de INST.DE QUIMICA DEL NOROESTE
Articulos de INST.DE QUIMICA DEL NOROESTE
Citación
Rey, Valentina; Gramajo Feijoo, Marcelo Eduardo; Gimenez, Rodrigo Esteban; Tuttolomondo, María Eugenia; Moran Vieyra, Faustino Eduardo; et al.; Kinetics and growth mechanism of the photoinduced synthesis of silver nanoparticles stabilized with lysozyme; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 172; 12-2018; 10-16
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