Artículo
Sperm sexing mediated by magnetic nanoparticles in donkeys, a preliminary in vitro study
Dominguez, Esteban Mauricio
; Moreno, Ayelen
; Ramírez Castex, Hernán; Flores Bragulat, Ana Paula; Ugaz, Cristian; Clemente, Henry; Giojalas, Laura Cecilia
; Losinno, Luis
Fecha de publicación:
06/2018
Editorial:
Elsevier Science Inc
Revista:
Journal of Equine Veterinary Science
ISSN:
0737-0806
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Preselection of sex before conception has been one of the objectives pursued by scientists and breeders for many years. The donkey milk industry has shown a greater commercial interest, since jennies’ demand is increasing nowadays. Therefore, to get a female animal, the oocyte must be fertilized with previously sexed semen. The current technique used for sperm sexing separates spermatozoa containing the X or Y chromosome based on each cell DNA content. However, this technique exposes spermatozoa to high-pressure speed flow, DNA fluorescent stain, and UV light, factors that may affect sperm quality. The aim of this study was to test a new technique to isolate spermatozoa carrying the X chromosome by means of magnetic nanoparticles (MNPs). Results show that the MNP technique is highly effective in select X spermatozoa, without affecting several physiological sperm parameters. In conclusion, the MNP technique provides an X sperm population with similar sperm physiological state than the control sample. The new sexing technology presented here can be mainly applied to improve the donkey's milk industry.
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Articulos(IIBYT)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Citación
Dominguez, Esteban Mauricio; Moreno, Ayelen; Ramírez Castex, Hernán; Flores Bragulat, Ana Paula; Ugaz, Cristian; et al.; Sperm sexing mediated by magnetic nanoparticles in donkeys, a preliminary in vitro study; Elsevier Science Inc; Journal of Equine Veterinary Science; 65; 6-2018; 123-127
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