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dc.contributor.author
Rosano, German Leandro  
dc.contributor.author
Ceccarelli, Eduardo Augusto  
dc.date.available
2016-12-05T20:14:51Z  
dc.date.issued
2014-04  
dc.identifier.citation
Rosano, German Leandro; Ceccarelli, Eduardo Augusto; Recombinant protein expression in Escherichia coli: advances and challenges; Frontiers; Frontiers in Microbiology; 5; 4-2014; 1-17; 172  
dc.identifier.uri
http://hdl.handle.net/11336/8821  
dc.description.abstract
Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies.We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Frontiers  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Expresión de Proteínas  
dc.subject
Proteínas Heterólogas  
dc.subject
Plásmidos de Expresión  
dc.subject
Escherichia Coli  
dc.subject
Recombinant Protein Expression  
dc.subject
Expression Plasmid  
dc.subject
Inclusion Bodies  
dc.subject
Affinity Tags  
dc.subject
E. Coli Expression Strains  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Recombinant protein expression in Escherichia coli: advances and challenges  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2016-11-24T19:37:13Z  
dc.identifier.eissn
1664-302X  
dc.journal.volume
5  
dc.journal.pagination
1-17; 172  
dc.journal.pais
Suiza  
dc.journal.ciudad
Lausanne  
dc.description.fil
Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina  
dc.description.fil
Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina  
dc.journal.title
Frontiers in Microbiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.frontiersin.org/Journal/Abstract.aspx?s=679&name=microbiotechnology,_ecotoxicology_and_bioremediation&ART_DOI=10.3389/fmicb.2014.00172  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fmicb.2014.00172  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4029002/  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://doaj.org/article/d515e752014c4329afbe7ac76820c696  

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