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Artículo

Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ

Domizi, Pablo DanielIcon ; Aoyama, Chieko; Banchio, Claudia Elena
Fecha de publicación: 04/2014
Editorial: Elsevier
Revista: Biochimica Et Biophysica Acta - Molecular And Cell Biology Of Lipids
ISSN: 1388-1981
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Neuronal differentiation is a complex process characterized by a halt in proliferation and extension of neurites from the cell body. This process is accompanied by changes in gene expression that mediate the redirection leading to neurite formation and function. Acceleration of membrane phospholipids synthesis is associated with neurite elongation, and phosphatidylcholine (PtdCho) is the major membrane phospholipid in mammalian cells. The transcription of two genes in particular encoding key enzymes in the CDP–choline pathway for PtdCho biosynthesis are stimulated; the Chka gene for choline kinase (CK) alpha isoform and the Pcyt1a gene for the CTP: phosphocholine cytidylyltransferase (CCT) alpha isoform. We report that the stimulation of CKα expression during retinoic acid (RA) induced differentiation depends on a promoter region that contains two CCAAT/Enhancerbinding Protein-β (C/EBPβ) sites. We demonstrate that during neuronal differentiation of Neuro-2a cells, RA induces Chka expression by a mechanism that involves ERK½ activation which triggers C/EBPβ expression. Elevated levels of C/EBPβ bind to the Chka proximal promoter (Box1) inducing CKα expression. In addition we identified a downstream sequence named Box2 which together with Box1 is required for the promoter to reach the full induction. This is the first elucidation of the mechanism by which the expression of Chka is coordinately regulated during neuronal differentiation.
Palabras clave: Chka Gene Promoter , Phosphatidylcholine , Neuritogenesis , Transcriptional Regulation , C/Ebpβ , Lipidos , Colina Kinasa
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/8816
URL: http://www.sciencedirect.com/science/article/pii/S1388198114000122
DOI: http://dx.doi.org/10.1016/j.bbalip.2014.01.007
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Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Domizi, Pablo Daniel; Aoyama, Chieko; Banchio, Claudia Elena; Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ; Elsevier; Biochimica Et Biophysica Acta - Molecular And Cell Biology Of Lipids; 1841; 4; 4-2014; 544-555
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