The reinforcement of potato cell wall as part of the phosphite-induced tolerance to UV-B radiation

Abstract

Phosphites (Phis), inorganic salts of phosphorous acid, have shown to be effective in protection of plants against biotic stress. Recently, we have described that potassium phosphite (KPhi) induces tolerance to UV-B radiation in potato. To counteract the harmful effect of UV radiation, plants accumulate UV-screening compounds, such as flavonoids, sinapate ester, and lignin. In previous work, we have shown an increase in guaiacol peroxidase (POD) activity in plants pretreated with KPhi and further exposed to UV-B radiation. In order to continue with this study, the expression of different enzymes and components involved in cell wall reinforcement were analyzed. An isoform of POD induced by KPhi was analyzed by isoelectric focusing and further identified as suberization-associated anionic peroxidase (POPA) by mass spectrometry. In addition, other enzymes participating in lignin biosynthesis, like caffeoyl-CoA O-methyltransferase (CCoAOMT), determined by accumulation of transcripts, and laccase activity, visualized in zymogrames, were increased by KPhi treatment previous to UV-B exposure. Further, the accumulations of extensin (EXT) transcripts and of conjugated polyamines (PAs) were increased by KPhi treatment previous to UV-B exposure. All these results suggest cell wall reinforcement in leaves due to KPhi pretreatment followed by UV-B exposure.