Artículo
Haloferax volcanii proteome response to deletion of a rhomboid protease gene
Costa, Mariana Inés
; Cerletti, Micaela
; Paggi, Roberto Alejandro; Trötschel, Christian; de Castro, Rosana Esther
; Poetsch, Ansgar; Gimenez, Maria Ines
Fecha de publicación:
05/01/2018
Editorial:
American Chemical Society
Revista:
Journal of Proteome Research
ISSN:
1535-3893
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Rhomboids are conserved intramembrane serine proteases involved in cell signaling processes. Their role in prokaryotes is scarcely known and remains to be investigated in Archaea. We previously constructed a rhomboid homologue deletion mutant (ΔrhoII) in Haloferax volcanii, which showed reduced motility, increased novobiocin sensitivity, and an N- glycosylation defect. To address the impact of rhoII deletion on H. volcanii physiology, the proteomes of mutant and parental strains were compared by shotgun proteomics. A total of 1847 proteins were identified (45.8% of H. volcanii predicted proteome), from which 103 differed in amount. Additionally, the mutant strain evidenced 99 proteins with altered electrophoretic migration, which suggested differential post-translational processing/modification. Integral membrane proteins that evidenced variations in concentration, electrophoretic migration, or semitryptic cleavage in the mutant were considered as potential RhoII targets. These included a PrsW protease homologue (which was less stable in the mutant strain), a predicted halocyanin, and six integral membrane proteins potentially related to the mutant glycosylation (S-layer glycoprotein, Agl15) and cell adhesion/motility (flagellin1, HVO-1153, PilA1, and PibD) defects. This study investigated for the first time the impact of a rhomboid protease on the whole proteome of an organism.
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Articulos(IIB)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Citación
Costa, Mariana Inés; Cerletti, Micaela; Paggi, Roberto Alejandro; Trötschel, Christian; de Castro, Rosana Esther; et al.; Haloferax volcanii proteome response to deletion of a rhomboid protease gene; American Chemical Society; Journal of Proteome Research; 17; 3; 5-1-2018; 961-977
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