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dc.contributor.author
Brugler, Mercer R.
dc.contributor.author
González Muñoz, Ricardo Enrique

dc.contributor.author
Tessler, Michael
dc.contributor.author
Rodríguez, Estefanía
dc.date.available
2019-11-05T19:42:13Z
dc.date.issued
2018-11
dc.identifier.citation
Brugler, Mercer R.; González Muñoz, Ricardo Enrique; Tessler, Michael; Rodríguez, Estefanía; An EPIC journey to locate single-copy nuclear markers in sea anemones; Wiley Blackwell Publishing, Inc; Zoologica Scripta; 47; 6; 11-2018; 756-776
dc.identifier.issn
0300-3256
dc.identifier.uri
http://hdl.handle.net/11336/88082
dc.description.abstract
Sea anemones (Order Actiniaria) are among the most diverse members of the subclass Hexacorallia and are an emerging model system. Unfortunately, defining species and creating robust phylogenies remain a serious challenge due to simple body plans that reduce the number of available morphological characters, slow mitochondrial sequence evolution and a complete absence of nuclear markers in the actiniarian molecular toolkit other than the ribosomal cistron. Defining species boundaries and phylogenetic relationships is imperative for advancing our understanding of sea anemone taxonomy and systematics. Herein, we utilized EPIC (exon-priming intron-crossing) primers, in addition to non-standard PCR profiling conditions, to obtain sequence data for seven nuclear introns located within Calmodulin, Calpain, Nck Associated Protein 1 Homolog, Pescadillo Homolog, Signal Recognition Particle 54-kDa Subunit, Transferase and Vacuolar ATP Synthase Subunit B. These new markers were employed to evaluate whether morphological variability in marginal tentacle protuberances is indicative of intraspecific variation or cryptic species in the shallow-water anemone Phymanthus crucifer. Variability within these nuclear introns was compared to mitochondrial 12S, 16S and cox3, and nuclear 18S and 28S. PCR products of all nuclear introns were cloned to determine copy number and the resulting variability among clones was consistent with single-copy markers. We also amplified and sequenced six of the seven introns in a sister species, Phymanthus loligo, to determine the degree of interspecific variation. In addition, we evaluate the applicability of a subset of these markers within Actinostola and conclude with a review of putative single-copy markers used in octocoral and scleractinian phylogenetics.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc

dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Actiniaria
dc.subject
Actinostola
dc.subject
EPIC primers
dc.subject
Hexacorallia
dc.subject
Phymanthus
dc.subject
ribosomal cistron
dc.subject.classification
Zoología, Ornitología, Entomología, Etología

dc.subject.classification
Ciencias Biológicas

dc.subject.classification
CIENCIAS NATURALES Y EXACTAS

dc.subject.classification
Biología Marina, Limnología

dc.subject.classification
Ciencias Biológicas

dc.subject.classification
CIENCIAS NATURALES Y EXACTAS

dc.title
An EPIC journey to locate single-copy nuclear markers in sea anemones
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-24T19:36:26Z
dc.journal.volume
47
dc.journal.number
6
dc.journal.pagination
756-776
dc.journal.pais
Reino Unido

dc.journal.ciudad
Londres
dc.description.fil
Fil: Brugler, Mercer R.. American Museum of Natural History; Estados Unidos. City University of New York; Estados Unidos
dc.description.fil
Fil: González Muñoz, Ricardo Enrique. Universidad Nacional Autónoma de México; México. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina; Argentina
dc.description.fil
Fil: Tessler, Michael. American Museum of Natural History; Estados Unidos
dc.description.fil
Fil: Rodríguez, Estefanía. American Museum of Natural History; Estados Unidos
dc.journal.title
Zoologica Scripta
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/epdf/10.1111/zsc.12309
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/zsc.12309
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