Artículo
Human recombinant fab fragment neutralizes shiga toxin type 2 cytotoxic effects in vitro and in vivo
Luz, Daniela; Amaral, María Marta
; Sacerdoti, Flavia
; Bernal, Alan Mauro; Quintilio, Wagner; Moro, Ana Maria; Palermo, Marina Sandra
; Ibarra, Cristina Adriana
; Piazza, Roxane Maria Fontes
![Icon](/themes/CONICETDigital/images/conicet.png)
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Fecha de publicación:
12/2018
Editorial:
MDPI
Revista:
Toxins
ISSN:
2072-6651
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Shiga toxin (Stx) producing Escherichia coli (STEC) is responsible for causing hemolytic uremic syndrome (HUS), a life-threatening thrombotic microangiopathy characterized by thrombocytopenia, hemolytic anemia, and acute renal failure after bacterially induced hemorrhagic diarrhea. Until now, there has been neither an effective treatment nor method of prevention for the deleterious effects caused by Stx intoxication. Antibodies are well recognized as affinity components of therapeutic drugs; thus, a previously obtained recombinant human FabC11:Stx2 fragment was used to neutralize Stx2 in vitro in a Vero cell viability assay. Herein, we demonstrated that this fragment neutralized, in a dose-dependent manner, the cytotoxic effects of Stx2 on human glomerular endothelial cells, on human proximal tubular epithelial cells, and prevented the morphological alterations induced by Stx2. FabC11:Stx2 protected mice from a lethal dose of Stx2 by toxin-antibody pre-incubation. Altogether, our results show the ability of a new encouraging molecule to prevent Stx-intoxication symptoms during STEC infection.
Palabras clave:
FAB
,
NEUTRALIZATION
,
PROTECTION
,
RECOMBINANT ANTIBODY FRAGMENT
,
SHIGA TOXIN
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IFIBIO HOUSSAY)
Articulos de INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Articulos de INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Articulos(IMEX)
Articulos de INST.DE MEDICINA EXPERIMENTAL
Articulos de INST.DE MEDICINA EXPERIMENTAL
Citación
Luz, Daniela; Amaral, María Marta; Sacerdoti, Flavia; Bernal, Alan Mauro; Quintilio, Wagner; et al.; Human recombinant fab fragment neutralizes shiga toxin type 2 cytotoxic effects in vitro and in vivo; MDPI; Toxins; 10; 12; 12-2018; 1-10
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