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dc.contributor.author
Chara, Osvaldo
dc.contributor.author
Borges, Augusto
dc.contributor.author
Milhiet, Pierre Emmanuel
dc.contributor.author
Nöllmann, Marcelo
dc.contributor.author
Cattoni, Diego I.
dc.date.available
2019-11-04T17:36:32Z
dc.date.issued
2018-12
dc.identifier.citation
Chara, Osvaldo; Borges, Augusto; Milhiet, Pierre Emmanuel; Nöllmann, Marcelo; Cattoni, Diego I.; Sequence-dependent catalytic regulation of the SpoIIIE motor activity ensures directionality of DNA translocation; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018
dc.identifier.issn
2045-2322
dc.identifier.uri
http://hdl.handle.net/11336/87917
dc.description.abstract
Transport of cellular cargo by molecular motors requires directionality to ensure proper biological functioning. During sporulation in Bacillus subtilis, directionality of chromosome transport is mediated by the interaction between the membrane-bound DNA translocase SpoIIIE and specific octameric sequences (SRS). Whether SRS regulate directionality by recruiting and orienting SpoIIIE or by simply catalyzing its translocation activity is still unclear. By using atomic force microscopy and single-round fast kinetics translocation assays we determined the localization and dynamics of diffusing and translocating SpoIIIE complexes on DNA with or without SRS. Our findings combined with mathematical modelling revealed that SpoIIIE directionality is not regulated by protein recruitment to SRS but rather by a fine-tuned balance among the rates governing SpoIIIE-DNA interactions and the probability of starting translocation modulated by SRS. Additionally, we found that SpoIIIE can start translocation from non-specific DNA, providing an alternative active search mechanism for SRS located beyond the exploratory length defined by 1D diffusion. These findings are relevant in vivo in the context of chromosome transport through an open channel, where SpoIIIE can rapidly explore DNA while directionality is modulated by the probability of translocation initiation upon interaction with SRS versus non-specific DNA.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DNA TRANSLOCATION
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SPORULATION
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MONTE CARLO MODELLING
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CHROMOSOME TRANSPORT
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Sequence-dependent catalytic regulation of the SpoIIIE motor activity ensures directionality of DNA translocation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-17T20:13:07Z
dc.journal.volume
8
dc.journal.number
1
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina
dc.description.fil
Fil: Borges, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina
dc.description.fil
Fil: Milhiet, Pierre Emmanuel. Université Montpellier II; Francia
dc.description.fil
Fil: Nöllmann, Marcelo. Université Montpellier II; Francia
dc.description.fil
Fil: Cattoni, Diego I.. Université Montpellier II; Francia
dc.journal.title
Scientific Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/s41598-018-23400-8
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-23400-8
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