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dc.contributor.author
Krolewiecki, Alejandro Javier  
dc.contributor.author
Koukounari, Artemis  
dc.contributor.author
Romano, Miryam de Los Angeles  
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Caro, Reynaldo N.  
dc.contributor.author
Scott, Alan L  
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Fleitas, Pedro Emanuel  
dc.contributor.author
Cimino, Rubén Oscar  
dc.contributor.author
Shiff, Clive J.  
dc.date.available
2019-10-31T19:41:31Z  
dc.date.issued
2018-06-01  
dc.identifier.citation
Krolewiecki, Alejandro Javier; Koukounari, Artemis; Romano, Miryam de Los Angeles; Caro, Reynaldo N.; Scott, Alan L; et al.; Transrenal DNA-based diagnosis of Strongyloides stercoralis (Grassi, 1879) infection: Bayesian latent class modeling of test accuracy; Public Library of Science; Neglected Tropical Diseases; 12; 6; 1-6-2018; 1-11; e0006550  
dc.identifier.issn
1935-2735  
dc.identifier.uri
http://hdl.handle.net/11336/87774  
dc.description.abstract
For epidemiological work with soil transmitted helminths the recommended diagnostic approaches are to examine fecal samples for microscopic evidence of the parasite. In addition to several logistical and processing issues, traditional diagnostic approaches have been shown to lack the sensitivity required to reliably identify patients harboring low-level infections such as those associated with effective mass drug intervention programs. In this context, there is a need to rethink the approaches used for helminth diagnostics. Serological methods are now in use, however these tests are indirect and depend on individual immune responses, exposure patterns and the nature of the antigen. However, it has been demonstrated that cell-free DNA from pathogens and cancers can be readily detected in patient’s urine which can be collected in the field, filtered in situ and processed later for analysis. In the work presented here, we employ three diagnostic procedures—stool examination, serology (NIE-ELISA) and PCR-based amplification of parasite transrenal DNA from urine–to determine their relative utility in the diagnosis of S. stercoralis infections from 359 field samples from an endemic area of Argentina. Bayesian Latent Class analysis was used to assess the relative performance of the three diagnostic procedures. The results underscore the low sensitivity of stool examination and support the idea that the use of serology combined with parasite transrenal DNA detection may be a useful strategy for sensitive and specific detection of low-level strongyloidiasis.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Public Library of Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
STRONGYLOIDES STERCORALIS  
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DIAGNOSTICS  
dc.subject
URINE  
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PCR  
dc.subject.classification
Enfermedades Infecciosas  
dc.subject.classification
Ciencias de la Salud  
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Transrenal DNA-based diagnosis of Strongyloides stercoralis (Grassi, 1879) infection: Bayesian latent class modeling of test accuracy  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-10-16T14:23:55Z  
dc.journal.volume
12  
dc.journal.number
6  
dc.journal.pagination
1-11; e0006550  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
San Francisco  
dc.description.fil
Fil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Koukounari, Artemis. Liverpool School of Tropical Medicine; Reino Unido  
dc.description.fil
Fil: Romano, Miryam de Los Angeles. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. University Johns Hopkins; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Caro, Reynaldo N.. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina  
dc.description.fil
Fil: Scott, Alan L. University Johns Hopkins; Estados Unidos  
dc.description.fil
Fil: Fleitas, Pedro Emanuel. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Cimino, Rubén Oscar. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Shiff, Clive J.. University Johns Hopkins; Estados Unidos  
dc.journal.title
Neglected Tropical Diseases  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pntd.0006550  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0006550