Artículo
Functional roles of C-terminal extension (CTE) of salt-dependent peptidase activity of the Natrialba magadii extracellular protease (NEP)
Marem, Alyne; Okamoto, Debora N.; Oliveira, Lilian C.G.; Ruiz, Diego M.; Paggi, Roberto Alejandro; Kondo, Marcia Y.; Gouvea, Iuri E.; Juliano, Maria A.; de Castro, Rosana Esther
; Juliano, Luiz; Icimoto, Marcelo Y.
Fecha de publicación:
07/07/2018
Editorial:
Elsevier Science
Revista:
International Journal of Biological Macromolecules
ISSN:
0141-8130
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon Natrialba magadii. Many extracellular proteases have been characterized from archaea to bacteria as adapted to hypersaline environments retaining function and stability until 4.0 M NaCl. As observed in other secreted halolysins, this stability can be related to the presence of a C-terminal extension (CTE) sequence. In the present work, we compared the biochemical properties of recombinant Nep protease with the truncated form at the 134 amino acids CTE (Nep∆CTE), that was more active in 4 M NaCl than the non-truncated wild type enzyme. Comparable to the wild type, Nep∆CTE protease is irreversibly inactivated at low salt solutions. The substrate specificity of the truncated Nep∆CTE was similar to that of wild type form as demonstrated by a combinatorial library of FRET substrates. The enzyme stability, the effect of different salts and the thermodynamics assays using different lengths of substrates demonstrated similarities between the two forms. Altogether, these data provide further information on the stability and structural determinants of halolysins under different salinities, especially concerning the enzymatic behavior.
Palabras clave:
Halophilic protease
,
Natrialba magadii
,
Serine protease
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Articulos(IIB)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Citación
Marem, Alyne; Okamoto, Debora N.; Oliveira, Lilian C.G.; Ruiz, Diego M.; Paggi, Roberto Alejandro; et al.; Functional roles of C-terminal extension (CTE) of salt-dependent peptidase activity of the Natrialba magadii extracellular protease (NEP); Elsevier Science; International Journal of Biological Macromolecules; 113; 7-7-2018; 1134-1141
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