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Artículo

Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis

Piguillem Palacios, Sofía VivianaIcon ; Ortega Sánchez, Francisco Gabriel; Raba, JulioIcon ; Messina, Germán AlejandroIcon ; Fernández Baldo, Martín AlejandroIcon
Fecha de publicación: 06/2018
Editorial: Elsevier Science
Revista: Microchemical Journal
ISSN: 0026-265X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Química Analítica

Resumen

The use of a biological marker as an adjunct for screening for invasive aspergillosis (IA) in high-risk patients is attractive, because it is noninvasive and may detect evidence of IA prior to the appearance of clinical signs and symptoms. Galactomannan (GMN) is a polysaccharide cell-wall component that is released by Aspergillus species. In the present work, an electrochemical microfluidic immunosensor for GMN detection was developed. Moreover, copper nanoparticles covered with polyvinylpyrrolidone (CuNPs-PVP) were synthesized, characterized and used in a microfluidic immunosensor based on the use of anti-GMN antibodies as a trapping agent. The concentration of trapped GMN is then electrochemically quantified by HRP-conjugated anti-GMN-antibody. HRP reacted with its enzymatic substrate in a redox process which resulted in the appearance of a current whose magnitude is directly proportional to the concentration of GMN. The immunosensor was validated by analyzing patient samples, and a good correlation with a commercial ELISA was obtained. This microfluidic immunosensor can be applied to the early diagnosis of IA.
Palabras clave: CLINICAL DIAGNOSIS , ELECTROCHEMICAL , GALACTOMANNAN , INVASIVE ASPERGILLOSIS , MICROFLUIDIC IMMUNOSENSOR , NANO-PLATFORM
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/87333
URL: https://www.sciencedirect.com/science/article/pii/S0026265X17313358
DOI: https://doi.org/10.1016/j.microc.2018.03.025
Colecciones
Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Piguillem Palacios, Sofía Viviana; Ortega Sánchez, Francisco Gabriel; Raba, Julio; Messina, Germán Alejandro; Fernández Baldo, Martín Alejandro; Development of a nanostructured electrochemical immunosensor applied to the early detection of invasive aspergillosis; Elsevier Science; Microchemical Journal; 139; 6-2018; 394-400
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