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Artículo

Molecular features of nonionic detergents involved in the binding kinetics and solubilization efficiency, as studied in model (Langmuir films) and biological (Erythrocytes) membranes

Casadei, Bruna Renata; Domingues, Cleyton Crepaldi; Clop, Eduardo MatiasIcon ; Couto, Verônica Muniz; Perillo, Maria AngelicaIcon ; de Paula, Eneida
Fecha de publicación: 01/06/2018
Editorial: Elsevier Science
Revista: Colloids and Surfaces B: Biointerfaces
ISSN: 0927-7765
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

The effect of the nonionic detergents Brij-98 and Brij-58 over human erythrocytes was studied through quantitative hemolysis and in Langmuir films. Hemolytic tests revealed that Brijs are stronger membrane solubilizers than Triton X-100 (TX-100), with effective detergent/lipid ratios of 0.18 and 0.37 for Brij-98 and Brij-58, respectively. Experiments with Langmuir films provided significant information on the kinetics and thermodynamics of detergent-membrane interaction. The adsorption (k a ) and desorption (k d ) rate constants of Brijs were lower than those of TX-100. In the case of k a , that is probably due to their larger hydrophilic head (with twice (20) the oxyethylene units of TX-100). As for the thermodynamic binding constant, the linear and longer hydrophobic acyl chains of Brijs favor their stabilization in-between the lipids, through London van der Waals forces. Consequently, K b,m values of Brij-98 (12,500 M −1 ) and Brij-58 (19,300 M −1 ) resulted higher than TX-100 (7500 M −1 ), in agreement with results from the hemolytic tests. Furthermore, Brij-58 binds with higher affinity than Brij-98 to bilayers and monolayers, despite its shorter (palmitic) hydrocarbon chain, showing that unsaturation restrains the detergent insertion into these environments. Our results provide significant information about the mechanism of interaction between Brijs and membranes, supporting their distinct solubilization effect.
Palabras clave: ERYTHROCYTE MEMBRANES , SOLUBILIZATION , MONOLAYERS , NONIONIC DETERGENTS , BRIJ
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/87057
URL: https://www.sciencedirect.com/science/article/pii/S0927776518301541
DOI: http://dx.doi.org/10.1016/j.colsurfb.2018.03.012
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Articulos(IIBYT)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Citación
Casadei, Bruna Renata; Domingues, Cleyton Crepaldi; Clop, Eduardo Matias; Couto, Verônica Muniz; Perillo, Maria Angelica; et al.; Molecular features of nonionic detergents involved in the binding kinetics and solubilization efficiency, as studied in model (Langmuir films) and biological (Erythrocytes) membranes; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 166; 1-6-2018; 152-160
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