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dc.contributor.author
Manzo, Ricardo Martín
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Ceruti, Roberto Julio
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Bonazza, Horacio
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Adriano, Wellington S.
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Sihufe, Guillermo Adrian
dc.contributor.author
Mammarella, Enrique José
dc.date.available
2019-10-23T01:11:12Z
dc.date.issued
2018-08
dc.identifier.citation
Manzo, Ricardo Martín; Ceruti, Roberto Julio; Bonazza, Horacio; Adriano, Wellington S.; Sihufe, Guillermo Adrian; et al.; Immobilization of Carboxypeptidase A into Modified Chitosan Matrixes by Covalent Attachment; Humana Press; Applied Biochemistry And Biotechnology; 185; 4; 8-2018; 1029-1043
dc.identifier.issn
0273-2289
dc.identifier.uri
http://hdl.handle.net/11336/87037
dc.description.abstract
Carboxypeptidase A (CPA) is a metalloexopeptidase that catalyzes the hydrolysis of the peptide bonds that are adjacent to the C-terminal end of a polypeptide chain. The enzyme preferentially cleaves over C-terminal L-amino acids with aromatic or branched side chains. This is of main importance for food industry because it can be employed for manufacturing functional foods from different protein sources with reduced hydrophobic amino acid content for patients with deficiencies in the absorption or digestion of the corresponding amino acids. In that way, strategies for effective multipoint covalent immobilization of CPA metalloenzyme on chitosan beads have been developed. The study of the ability to produce several chemical modifications on chitosan molecules before, during and after its coagulation to form carrier beads lead in a protective effect of the polymer matrix. The chemical modification of chitosan through the use of an N-alkylation strategy produced the best derivatives. N-alkyl chitosan derivative beads with D-fructose presented values of 0.86 for immobilization yield, 314.6 IU g−1 bead for initial activity of biocatalyst and were 5675.64-fold more stable than the free enzyme at 55 °C. Results have shown that these derivatives would present a potential technological application in hydrolytic processes due to both their physical properties, such as low swelling capacity, reduced metal chelation ability and bulk mesoporosity, and increased operational stability when compared with soluble enzyme.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Humana Press
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CARBOXYPEPTIDASE A
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CHITOSAN
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IMMOBILIZATION
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MULTIPOINT COVALENT ATTACHMENT
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SWELLING
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Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
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Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
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Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
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Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
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Química Orgánica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Immobilization of Carboxypeptidase A into Modified Chitosan Matrixes by Covalent Attachment
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-18T18:05:49Z
dc.journal.volume
185
dc.journal.number
4
dc.journal.pagination
1029-1043
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Oregon
dc.description.fil
Fil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Ceruti, Roberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Bonazza, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
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Fil: Adriano, Wellington S.. Universidad Federal de Campina Grande; Brasil
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Fil: Sihufe, Guillermo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.journal.title
Applied Biochemistry And Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s12010-018-2708-4
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12010-018-2708-4
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