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Artículo

Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6

Bott, EmanuelIcon ; Carneiro, Alan B.; Gimenez, GuadalupeIcon ; López, María GabrielaIcon ; Lammel, Estela MaríaIcon ; Atella, Georgia C.; Bozza, Patricia T.; Belaunzarán, María LauraIcon
Fecha de publicación: 05/2018
Editorial: Frontiers Media SA
Revista: Frontiers in Cellular and Infection Microbiology
ISSN: 2235-2988
e-ISSN: 2235-2988
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Lipids from microorganisms are ligands of Toll like receptors (TLRs) and modulate the innate immune response. Herein, we analyze in vitro the effect of total lipid extracts from Trypanosoma cruzi amastigotes of RA and K98 strains (with polar biological behavior) on the induction of the inflammatory response and the involvement of TLRs in this process. We demonstrated that total lipid extracts from both strains induced lipid body formation, cyclooxygenase-2 expression and TNF-a and nitric oxide release in macrophages, as well as NF-κB activation and IL-8 release in HEK cells specifically through a TLR2/6 dependent pathway. We also evaluated the inflammatory response induced by total lipid extracts obtained from lysed parasites that were overnight incubated to allow the action of parasite hydrolytic enzymes, such as Phospholipase A1, over endogenous phospholipids. After incubation, these total lipid extracts showed a significantly reduced pro-inflammatory response, which could be attributed to the changes in the content of known bioactive lipid molecules like lysophospholipids and fatty acids, here reported. Moreover, analyses of total fatty acids in each lipid extract were performed by gas chromatography-mass spectrometry. Our results indicate a relevant role of T. cruzi lipids in the induction of a pro-inflammatory response through the TLR2/6 pathway that could contribute to the modulation of the immune response and host survival.
Palabras clave: LIPIDS , PHOSPHOLIPASE A1 , PRO-INFLAMMATORY , TLR2/6 , TRYPANOSOMA CRUZI
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/86949
URL: http://journal.frontiersin.org/article/10.3389/fcimb.2018.00151/full
DOI: https://doi.org/10.3389/fcimb.2018.00151
Colecciones
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Bott, Emanuel; Carneiro, Alan B.; Gimenez, Guadalupe; López, María Gabriela; Lammel, Estela María; et al.; Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6; Frontiers Media SA; Frontiers in Cellular and Infection Microbiology; 8; 151; 5-2018; 1-14
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