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dc.contributor.author
Sturme, Mark H.J.
dc.contributor.author
Gong, Yanhai
dc.contributor.author
Heinrich, Josué Miguel
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dc.contributor.author
Klok, Anne J.
dc.contributor.author
Eggink, Gerrit
dc.contributor.author
Wang, Dongmei
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Xu, Jian
dc.contributor.author
Wijffels, Rene H.
dc.date.available
2019-10-22T16:59:46Z
dc.date.issued
2018-07
dc.identifier.citation
Sturme, Mark H.J.; Gong, Yanhai; Heinrich, Josué Miguel; Klok, Anne J.; Eggink, Gerrit; et al.; Transcriptome analysis reveals the genetic foundation for the dynamics of starch and lipid production in Ettlia oleoabundans; Elsevier B.V.; Algal Research; 33; 7-2018; 142-155
dc.identifier.issn
2211-9264
dc.identifier.uri
http://hdl.handle.net/11336/86888
dc.description.abstract
The oleaginous microalga Ettlia oleoabundans accumulates both starch and lipids to high levels under stress conditions such as nitrogen starvation (N−). To steer biosynthesis towards starch or lipids only, it is important to understand the regulatory mechanisms involved. Here physiological and transcriptional changes under nitrogen starvation were analysed in controlled flat-panel photobioreactors at both short and long time-scales. Starch accumulation was transient and occurred rapidly within 24 h upon starvation, while lipid accumulation was gradual and reached a maximum after 4 days. The major fraction of accumulated lipids was composed of de novo synthesized neutral lipids - triacylglycerides (TAG) - and was characterized by a decreased composition of the polyunsaturated fatty acids (PUFAs) C18:3 and C16:3 and an increased composition of the mono-unsaturated (MUFAs) and saturated (SFAs) fatty acids C18:1/C16:1 and C18:0/C16:0, respectively. RNA-sequencing revealed that starch biosynthesis and degradation genes show different expression dynamics from lipid biosynthesis ones. An immediate rapid increase in starch synthetic transcripts was followed by an increase in starch degrading transcripts and a decrease in the starch synthetic ones. In contrast, increased gene expression for fatty acid and TAG synthesis was initiated later and occurred more gradually. Expression of several fatty acid desaturase (FAD) genes was decreased upon starvation, which corresponds to the observed changes to higher levels of MUFAs and SFAs. Moreover, several homologs of transcription regulators that were implicated in controlling starch and lipid metabolism in other microalgae showed differential gene expression and might be key regulators of starch and lipid metabolism in E. oleoabundans as well. Our data provide insights into the genetic foundation of starch and lipid metabolism in E. oleoabundans under nitrogen starvation and should facilitate metabolic engineering towards tailored strains with desired storage compound composition.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier B.V.
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CARBON-PARTITIONING
dc.subject
LIPIDS
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MICROALGAE
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NITROGEN STARVATION
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STARCH
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TRANSCRIPTOME
dc.subject.classification
Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
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dc.subject.classification
Biotecnología Industrial
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Transcriptome analysis reveals the genetic foundation for the dynamics of starch and lipid production in Ettlia oleoabundans
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-18T18:06:15Z
dc.journal.volume
33
dc.journal.pagination
142-155
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsteram
dc.description.fil
Fil: Sturme, Mark H.J.. University of Agriculture Wageningen; Países Bajos
dc.description.fil
Fil: Gong, Yanhai. Qingdao Institute Of Bioenergy And Bioprocess Technolog; China
dc.description.fil
Fil: Heinrich, Josué Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
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Fil: Klok, Anne J.. University of Agriculture Wageningen; Países Bajos
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Fil: Eggink, Gerrit. University of Agriculture Wageningen; Países Bajos
dc.description.fil
Fil: Wang, Dongmei. Qingdao Institute Of Bioenergy And Bioprocess Technolog; China
dc.description.fil
Fil: Xu, Jian. Qingdao Institute Of Bioenergy And Bioprocess Technolog; China
dc.description.fil
Fil: Wijffels, Rene H.. University of Agriculture Wageningen; Países Bajos
dc.journal.title
Algal Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S2211926417311451
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.algal.2018.05.004
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