Artículo
Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration
Martín González, Natalia; Guérin Darvas, Sofía M.; Durana, Aritz; Marti, Gerardo Anibal
; Guérin, Diego M. A.; de Pablo, Pedro J.
Fecha de publicación:
06/2018
Editorial:
IOP Publishing
Revista:
Journal of Physics: Condensed Matter
ISSN:
0953-8984
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Even though viruses evolve mainly in liquid milieu, their horizontal transmission routesoften include episodes of dry environment. Along their life cycle, some insect viruses, suchas viruses from the Dicistroviridae family, withstand dehydrated conditions with presentlyunknown consequences to their structural stability. Here, we use atomic force microscopy tomonitor the structural changes of viral particles of Triatoma virus (TrV) after desiccation. Ourresults demonstrate that TrV capsids preserve their genome inside, conserving their heightafter exposure to dehydrating conditions, which is in stark contrast with other viruses thatexpel their genome when desiccated. Moreover, empty capsids (without genome) resultedin collapsed particles after desiccation. We also explored the role of structural ions in thedehydration process of the virions (capsid containing genome) by chelating the accessiblecations from the external solvent milieu. We observed that ion suppression helps to keep thevirus height upon desiccation. Our results show that under drying conditions, the genomeof TrV prevents the capsid from collapsing during dehydration, while the structural ions areresponsible for promoting solvent exchange through the virion wall.
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Identificadores
Colecciones
Articulos(CEPAVE)
Articulos de CENTRO DE EST.PARASITOL.Y DE VECTORES (I)
Articulos de CENTRO DE EST.PARASITOL.Y DE VECTORES (I)
Citación
Martín González, Natalia; Guérin Darvas, Sofía M.; Durana, Aritz; Marti, Gerardo Anibal; Guérin, Diego M. A.; et al.; Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration; IOP Publishing; Journal of Physics: Condensed Matter; 30; 10; 6-2018; 1-9
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