Mostrar el registro sencillo del ítem
dc.contributor.author
Zimaro, Tamara
dc.contributor.author
Thomas, Ludivine
dc.contributor.author
Marondedze, Claudius
dc.contributor.author
Sgro, Germán Gustavo
dc.contributor.author
Garofalo, Cecilia Graciela
dc.contributor.author
Ficarra, Florencia Andrea
dc.contributor.author
Gehring, Chris
dc.contributor.author
Ottado, Jorgelina
dc.contributor.author
Gottig Schor, Natalia
dc.date.available
2016-12-02T14:49:44Z
dc.date.issued
2014-04
dc.identifier.citation
Zimaro, Tamara; Thomas, Ludivine; Marondedze, Claudius; Sgro, Germán Gustavo; Garofalo, Cecilia Graciela; et al.; The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation; Biomed Central; Bmc Microbiology; 14; 96; 4-2014; 96-111
dc.identifier.issn
1471-2180
dc.identifier.uri
http://hdl.handle.net/11336/8659
dc.description.abstract
Background: Several bacterial plant pathogens colonize their hosts through the secretion of effector proteins by a Type III protein secretion system (T3SS). The role of T3SS in bacterial pathogenesis is well established but whether this system is involved in multicellular processes, such as bacterial biofilm formation has not been elucidated. Here, the phytopathogen Xanthomonas citri subsp. citri (X. citri) was used as a model to gain further insights about the role of the T3SS in biofilm formation.
Results: The capacity of biofilm formation of different X. citri T3SS mutants was compared to the wild type strain and it was observed that this secretion system was necessary for this process. Moreover, the T3SS mutants adhered proficiently to leaf surfaces but were impaired in leaf-associated growth. A proteomic study of biofilm cells showed that the lack of the T3SS causes changes in the expression of proteins involved in metabolic processes, energy generation, exopolysaccharide (EPS) production and bacterial motility as well as outer membrane proteins. Furthermore, EPS production and bacterial motility were also altered in the T3SS mutants.
Conclusions: Our results indicate a novel role for T3SS in X. citri in the modulation of biofilm formation. Since this process increases X. citri virulence, this study reveals new functions of T3SS in pathogenesis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Biomed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Xanthomonas Citri Subsp Citri
dc.subject
Biofilm
dc.subject
T3ss
dc.subject
Proteomics
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-24T19:37:41Z
dc.journal.volume
14
dc.journal.number
96
dc.journal.pagination
96-111
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Zimaro, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Thomas, Ludivine. King Abdullah University Of Science And Technology;
dc.description.fil
Fil: Marondedze, Claudius. King Abdullah University Of Science And Technology;
dc.description.fil
Fil: Sgro, Germán Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Garofalo, Cecilia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Ficarra, Florencia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Gehring, Chris. King Abdullah University Of Science And Technology;
dc.description.fil
Fil: Ottado, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Gottig Schor, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.journal.title
Bmc Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-14-96
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/1471-2180-14-96
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24742141/
Archivos asociados