Effect of Use Pre-Synch + Ovsynch Protocols on the Pregnancy of the Buffalo Rodeo of the Argentinean NEA

Abstract

A low intensity of homosexual behavior in buffaloes causes difficulties in the heat detection, subsequently impedes the implementation of artificial insemination. In cattle, there are several hormonal treated protocols for synchronizing ovulation followed by fixed-time artificial insemination (FTAI). Although synchronizing ovulation protocols and FTAI were extensively used in cattle, but very few reports were found using this approach in Argentinean rodeo buffaloes. The aim of the research was to evaluate the efficiency of using two different protocols for synchronizing ovulation, followed by FTAI. Conception rates of the animals receiving different hormonally treated regimes were reported. 320 female Argentinean rodeo buffaloes were randomly divided into two treatment groups. In group I: 190 animals were subjected to the Pre-Synch protocol where 150 mg of PGF2α was injectected intramuscularly twelve days prior to the first GnRH injection (50 mg synthetic GnRH) and designated as day 0, followed by IM injection of PGF22α, 7 day later (day7), the second injection of GnRH was administered two days later (day 9). In group 2, 130 animals were treated with standard OvSynch protocol. All the animals received FTAI at 16 h before the last injections of GnRH. Pregnancies were confirmed by ultrasonography 38 days before FTAI. Conception rates for the animals in group I and II were 55.8% and 52.3% respectively with overall conception rate of 54.4%. No statistical differences were found between the treatment groups (p>0.05), however numeric differences were observed.