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dc.contributor.author
Debernardi, Juan Manuel
dc.contributor.author
Mecchia, Martin Alejandro
dc.contributor.author
Vercruyssen, Liesbeth
dc.contributor.author
Smaczniak, Cezary
dc.contributor.author
Kaufmann, Kerstin
dc.contributor.author
Inze, Dirk
dc.contributor.author
Rodriguez Virasoro, Ramiro Esteban
dc.contributor.author
Palatnik, Javier Fernando
dc.date.available
2016-12-01T21:25:03Z
dc.date.issued
2014-08
dc.identifier.citation
Debernardi, Juan Manuel; Mecchia, Martin Alejandro; Vercruyssen, Liesbeth; Smaczniak, Cezary; Kaufmann, Kerstin; et al.; Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity; Wiley; Plant Journal; 79; 3; 8-2014; 413-426
dc.identifier.issn
0960-7412
dc.identifier.uri
http://hdl.handle.net/11336/8638
dc.description.abstract
The growth-regulating factors (GRFs) are plant-specific transcription factors. They form complexes with GRF-interacting factors (GIFs), a small family of transcriptional co-activators. In Arabidopsis thaliana, seven out of the nine GRFs are controlled by microRNA miR396. Analysis of Arabidopsis plants carrying a GRF3 allele insensitive to miR396 revealed a strong boost in the number of cells in leaves, which was further enhanced synergistically by an additional increase of GIF1 levels. Genetic experiments revealed that GRF3 can still increase cell number in gif1 mutants, albeit to a much lesser extent. Genome-wide transcript profiling indicated that the simultaneous increase of GRF3 and GIF1 levels causes additional effects in gene expression compared to either of the transgenes alone. We observed that GIF1 interacts in vivo with GRF3, as well as with chromatin-remodeling complexes, providing a mechanistic explanation for the synergistic activities of a GRF3?GIF1 complex. Interestingly, we found that, in addition to the leaf size, the GRF system also affects the organ longevity. Genetic and molecular analysis revealed that the functions of GRFs in leaf growth and senescence can be uncoupled, demonstrating that the miR396-GRF-GIF network impinges on different stages of leaf development. Our results integrate the post-transcriptional control of the GRF transcription factors with the progression of leaf development.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Mir396
dc.subject
Growth-Regulating Factor
dc.subject
Grf-Interacting Factors
dc.subject
An3
dc.subject
Proliferation
dc.subject
Senescence
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-24T19:37:47Z
dc.identifier.eissn
1365-313X
dc.journal.volume
79
dc.journal.number
3
dc.journal.pagination
413-426
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Debernardi, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Mecchia, Martin Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Vercruyssen, Liesbeth. University Of Ghent. Faculty Of Sciences. Department Of Plant Systems Biology; Bélgica
dc.description.fil
Fil: Smaczniak, Cezary. University Of Amsterdam. Department of Molecular Biology; Países Bajos
dc.description.fil
Fil: Kaufmann, Kerstin. University Of Amsterdam. Department of Molecular Biology; Países Bajos
dc.description.fil
Fil: Inze, Dirk. University Of Ghent. Faculty Of Sciences. Department Of Plant Systems Biology; Bélgica
dc.description.fil
Fil: Rodriguez Virasoro, Ramiro Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.journal.title
Plant Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/tpj.12567/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http:/dx.doi.org/10.1111/tpj.12567
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