Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

TAG synthesis and storage under osmotic stress. A requirement for preserving membrane homeostasis in renal cells

Weber, KarenIcon ; Casali, Cecilia IreneIcon ; Gaveglio, Virginia LucíaIcon ; Pasquaré, Susana Juana; Morel Gómez, Emanuel DarioIcon ; Parra, Leandro GastónIcon ; Erjavec, Luciana CeciliaIcon ; Perazzo, Cecilia; Fernandez, Maria del CarmenIcon
Fecha de publicación: 14/09/2018
Editorial: Elsevier Science
Revista: Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids
ISSN: 1388-1981
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Hyperosmolarity is a controversial signal for renal cells. It can induce cell stress or differentiation and both require an active lipid metabolism. We showed that hyperosmolarity upregulates phospholipid (PL) de novo synthesis in renal cells. PL synthesis requires fatty acids (FA), usually stored as triglycerides (TAG). PL and TAG de novo synthesis utilize the same initial biosynthetic route: sn-glycerol 3P (G3P) → phosphatidic acid (PA) → diacylglycerol (DAG). In the present work, we evaluate how such pathway contributes to PL and TAG synthesis in renal cells subjected to hyperosmolarity. Our results show an increase in PA and DAG formation under hyperosmotic conditions; augmented DAG production, due to lipin enzyme activity, lead to the increase of both TAG and PL. However, at early stages (24 and 48 h), most of the de novo synthesized DAG was directed to PL synthesis; longer treatments downregulated PL synthesis and the DAG formed was mainly driven to TAG synthesis. Hyperosmolarity induced ACC and FASN transcription which mediated FA de novo synthesis. New FA molecules were stored in TAG. Silencing experiments revealed that hyperosmotic-induction of lipin-1 and -2 was mediated by SREBP1. Interestingly, SREBP1 knockdown also dropped SREBP2, indicating a modulatory action between both isoforms. Impairing SREBP activity leads to a decline in TAG levels but not PL. Membrane homeostasis is maintained through the adequate PL synthesis and renewal and constitute a protective mechanism against hyperosmolarity. The present data reveal the relevance of TAG synthesis and storage for PL synthesis in renal cells.
Palabras clave: DGAT , HYPEROSMOLARITY , LIPIN , PHOSPHOLIPID SYNTHESIS , SREBP , TRIGLYCERIDE STORAGE
Ver el registro completo
 
Archivos asociados
Thumbnail
 
Tamaño: 9.386Mb
Formato: PDF
.
Descargar
Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/86243
DOI: http://dx.doi.org/10.1016/j.bbalip.2018.06.012
URL: https://www.sciencedirect.com/science/article/pii/S1388198118301355
Colecciones
Articulos(INIBIBB)
Articulos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Weber, Karen; Casali, Cecilia Irene; Gaveglio, Virginia Lucía; Pasquaré, Susana Juana; Morel Gómez, Emanuel Dario; et al.; TAG synthesis and storage under osmotic stress. A requirement for preserving membrane homeostasis in renal cells; Elsevier Science; Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids; 1863; 9; 14-9-2018; 1108-1120
Compartir
Altmétricas
 
Estadísticas
Visualizaciones: 95
Descargas: 325

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • Twitter
  • Instagram
  • YouTube
  • Sound Cloud

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

Ministerio
https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES