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dc.contributor.author
Baque, Maria Alejandra
dc.contributor.author
Gioria, Verónica Viviana
dc.contributor.author
Micheloud, Gabriela Analia
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Casado, Nieves Maria Claudia
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Claus, Juan Daniel
dc.contributor.author
Gennaro, Ana Maria
dc.date.available
2019-10-15T13:26:40Z
dc.date.issued
2018-11
dc.identifier.citation
Baque, Maria Alejandra; Gioria, Verónica Viviana; Micheloud, Gabriela Analia; Casado, Nieves Maria Claudia; Claus, Juan Daniel; et al.; Spin label EPR suggests the presence of cholesterol rich domains in cultured insect cell membranes; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 505; 4; 11-2018; 1038-1042
dc.identifier.issn
0006-291X
dc.identifier.uri
http://hdl.handle.net/11336/85862
dc.description.abstract
Different spin labels were incorporated to the membranes of cultured insect UFL-AG-286 cells in order to characterize their physical properties by Electron Paramagnetic Resonance spectroscopy (EPR). The spectrum of the spin label 12-SASL incorporated to cell membranes was similar as those obtained in membrane model systems composed of eggPC/cholesterol. However, the spectrum of the spin label CSL, chemically related to cholesterol, was drastically different in the two systems. Interestingly, when cell cholesterol content was reduced using methyl beta cyclodextrin, an EPR spectrum similar to those of model membranes was obtained. The analysis of these experiments suggests the existence of cholesterol rich regions in UFL-AG-286 cell membranes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CHOLESTANE SPIN LABEL
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CHOLESTEROL
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CULTURED INSECT CELLS
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LIPID RAFTS
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MEMBRANE FLUIDITY
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MEMBRANE NANODOMAINS
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Spin label EPR suggests the presence of cholesterol rich domains in cultured insect cell membranes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-10T14:59:25Z
dc.journal.volume
505
dc.journal.number
4
dc.journal.pagination
1038-1042
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Baque, Maria Alejandra. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina
dc.description.fil
Fil: Gioria, Verónica Viviana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Virologia; Argentina
dc.description.fil
Fil: Micheloud, Gabriela Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
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Fil: Casado, Nieves Maria Claudia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina
dc.description.fil
Fil: Claus, Juan Daniel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Virologia; Argentina
dc.description.fil
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Física del Litoral. Universidad Nacional del Litoral. Instituto de Física del Litoral; Argentina
dc.journal.title
Biochemical and Biophysical Research Communications
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0006291X18321478
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbrc.2018.10.011
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