Artículo
A colorimetric method for the assay of ADP-glucose pyrophosphorylase
Fusari, Corina; Demonte, Ana María Magdalena; Figueroa, Carlos Maria
; Aleanzi, Mabel Cristina; Iglesias, Alberto Alvaro
Fecha de publicación:
05/2006
Editorial:
Academic Press Inc Elsevier Science
Revista:
Analytical Biochemistry
ISSN:
0003-2697
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The purpose of the current work was to develop a relatively simple method to assay ADPGlcPPase activity having high sensitivity, accuracy, and reliability. We sought a procedure allowing the assay in both of the reaction directions, based on a technique suitable for the screening of numerous samples. The method quantifies inorganic orthophosphate released from the specific hydrolysis of the enzyme activity products. For ADPGlc synthesis, the method measures Pi after hydrolysis of PPi by inorganic pyrophosphatase. Pyrophosphorolysis is assayed by determining Pi derived from CF1-ATPase-mediated hydrolysis of ATP. Pi dosage is performed by the technique based in the formation of a phosphomolybdate?Malachite Green complex [14,15]. We optimized the procedure to a microscale grade, reaching convenient sensitivity and the possibility of automation, by using a microplate (multiwell) absorbance reader
Palabras clave:
ADP-GLUCOSE PYROPHOSPHORYLASE
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Articulos(IAL)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Citación
Fusari, Corina; Demonte, Ana María Magdalena; Figueroa, Carlos Maria; Aleanzi, Mabel Cristina; Iglesias, Alberto Alvaro; A colorimetric method for the assay of ADP-glucose pyrophosphorylase; Academic Press Inc Elsevier Science; Analytical Biochemistry; 352; 1; 5-2006; 145-147
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