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dc.contributor.author
Suarez, Maria Fernanda  
dc.contributor.author
Piqueras, Maria Carmen  
dc.contributor.author
Correa, Leandro Javier  
dc.contributor.author
Esposito, Evangelina  
dc.contributor.author
Barros Centeno, María Fernanda  
dc.contributor.author
Bhattacharya, Sanjoy K.  
dc.contributor.author
Urrets Zavalía, Julio Alberto  
dc.contributor.author
Serra, Horacio Marcelo  
dc.date.available
2019-10-11T18:59:08Z  
dc.date.issued
2017-11  
dc.identifier.citation
Suarez, Maria Fernanda; Piqueras, Maria Carmen; Correa, Leandro Javier; Esposito, Evangelina; Barros Centeno, María Fernanda; et al.; Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Biochemistry; 118; 11; 11-2017; 3920-3931  
dc.identifier.issn
0730-2312  
dc.identifier.uri
http://hdl.handle.net/11336/85766  
dc.description.abstract
Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (e.g.,, contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile (phosphatidylcholine [PC] and phosphatidylserine [PS]) in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford's method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK's corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley-liss, Div John Wiley & Sons Inc  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
CDK  
dc.subject
CORNEA  
dc.subject
PHOSPHATIDYLCHOLINE  
dc.subject
PHOSPHATIDYLSERINE  
dc.subject
PHOSPHOLIPIDS  
dc.subject
TANDEM MASS SPECTROMETRY  
dc.subject.classification
Otras Ciencias de la Salud  
dc.subject.classification
Ciencias de la Salud  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-09-24T15:30:54Z  
dc.journal.volume
118  
dc.journal.number
11  
dc.journal.pagination
3920-3931  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Hoboken  
dc.description.fil
Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina  
dc.description.fil
Fil: Piqueras, Maria Carmen. University of Miami; Estados Unidos  
dc.description.fil
Fil: Correa, Leandro Javier. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina  
dc.description.fil
Fil: Esposito, Evangelina. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina  
dc.description.fil
Fil: Barros Centeno, María Fernanda. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina  
dc.description.fil
Fil: Bhattacharya, Sanjoy K.. University of Miami; Estados Unidos  
dc.description.fil
Fil: Urrets Zavalía, Julio Alberto. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina  
dc.description.fil
Fil: Serra, Horacio Marcelo. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina  
dc.journal.title
Journal of Cellular Biochemistry  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jcb.26045  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.26045  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603377/